Metabolic, immunologic, and histopathologic responses on premetamorphic American bullfrog (Lithobates catesbeianus) following exposure to lithium and selenium

Autor(es)

Pinto-Vidal, Felipe Augusto ^{[1, 2, 3]} ; Carvalho, Cleoni dos Santos ^[4] ; Abdalla, Fabio Camargo ^[5] ; Ceschi-Bertoli, Leticia ^[5] ; Moraes Utsunomiya, Heidi Samantha ^[4] ; da Silva, Renan Henrique ^[6] ; Salla, Raquel Fernanda ^[7] ; Jones-Costa, Monica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Lab Physiol Conservat LaFisC, Sorocaba, SP - Brazil

^[2] Masaryk Univ, Fac Sci, RECETOX, Kamenice 753-5, Pavil A29, Brno 62500 - Czech Republic

^[3] Univ Fed Sao Carlos, Postgrad Program Biotechnol & Environm Monitoring, Sorocaba, SP - Brazil

^[4] Univ Fed Sao Carlos, Lab Biochem & Microbiol LaBioM, Sorocaba, SP - Brazil

^[5] Univ Fed Sao Carlos, Lab Struct & Funct Biol LaBEF, Sorocaba, SP - Brazil

^[6] Fed Univ Southern Frontier UFFS, Realeza, Parana - Brazil

^[7] Univ Estadual Campinas, Lab Nat Hist Brazilian Amphibians LaHNAB, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 270, FEB 1 2021.
• Citações Web of Science: 0

Resumo

The presence of chemicals and the destruction of freshwater habitats have been addressed as one of the reasons for the decline in the amphibians' populations worldwide. Considering the threat that these animals have been suffering in tropical regions, the present study tested if the Brazilian legislation, concerning the permissive levels of lithium and selenium in water bodies and effluents, warrants the protection of aquatic life. To do so, we assessed the metabolic, immunologic, and histopathologic alterations in liver samples of American bullfrog (Lithobates catesbeianus), at the premetamorphic stage, through biomarkers indicative of general energetic status, i.e., glucose, lipid, and protein metabolism using biochemical and histochemical approaches. The immunologic responses were assessed by the quantification of melanomacrophage centres (MMCs); the histopathologic evaluation of the liver sections was also performed. The assay was carried out over 21 days with two periods of sampling (after 7 and 21 days) to assess the effects of exposure over time. The animals were exposed to the considered safe levels of lithium (2.5 mg L-1) and selenium (10 mg L-1), both, isolated and mixed. The exposed animals showed alterations in glucose and lipid metabolism throughout the experiment. The intense presence of MMCs and histopathological responses are compatible with hepatotoxicity. The toxicity expressed by the employed animal model indicates that the Brazilian environmental legislation for the protection of aquatic life needs to be updated. With this study, we intend to provide data for better environmental policies and bring attention to sublethal effects triggered by the presence of contaminants in the aquatic environment. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/57712-4 - Instituto Nacional de Pesquisas em Fisiologia Comparada
• Beneficiário: Augusto Shinya Abe
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/03653-6 - Sixth International Conference on Environmental Management, Engineering, Planning and Economics (CEMEPE) and to the SECOTOX
• Beneficiário: Fábio Camargo Abdalla
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 11/50752-3 - Biomarcadores de exposicao a metais em girinos de ra-touro.
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular