| Texto completo | |
| Autor(es): |
Martins, Bruna Rodrigues
[1]
;
Pinto, Thais Silva
[1]
;
da Costa Fernandes, Celio Junior
[1]
;
Bezerra, Fabio
[1]
;
Zambuzzi, Willian Fernando
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] UNESP Sao Paulo State Univ, Dept Chem & Biol Sci, Inst Biosci Botucatu, Botucatu, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 1 JAN 27 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Although osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces {[}presenting or not dual-acid etching (DAE)] up to 72h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. (AU) | |
| Processo FAPESP: | 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo |
| Beneficiário: | Willian Fernando Zambuzzi |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 19/09140-6 - Efeito do meio enriquecido com titânio no comportamento de células endoteliais submetidas ao modelo de shear-stress: análise de vias de adesão, sobrevivência e angiogênese |
| Beneficiário: | Bruna Rodrigues Martins |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |