Peptide Profile of Zebrafish Brain in a 6-OHDA-Induced Parkinson Model

Autor(es)

Fiametti, Louise Oliveira ^[1] ; Correa, Claudia Neves ^{[1, 2]} ; Castro, Leandro Mantovani de ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Biosci Inst, BR-11330900 Sao Vicente - Brazil

^[2] Sao Paulo State Univ UNESP, Biosci Inst, Biodivers Coastal Environm Postgrad Program, Sao Vicente - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZEBRAFISH; v. 18, n. 1 FEB 2021.
• Citações Web of Science: 0

Resumo

Parkinson's disease (PD) is a chronic neurodegenerative disorder mainly attributed to the progressive loss of dopaminergic neurons in the substantia nigra, which leads to uncontrolled voluntary movements causing tremors, postural instability, joint stiffness, and speech and locomotion difficulties, among other symptoms. Previous studies have shown the participation of specific peptides in neurodegenerative diseases. In this context, the present work analyzed changes in the peptide profile in zebrafish brain induced to parkinsonian conditions with 6-hydroxydopamine, using isotopic labeling techniques plus mass spectrometry. These analyses allowed the relative quantitation and identification of 118 peptides. Of these, nine peptides showed significant changes, one peptide was increased and eight decreased. The most altered sequences were fragment of cytosolic and extracellular proteins related to lipid metabolism and dynamic cytoskeleton. These results open new perspectives of study about the function of peptides in PD. (AU)

• Processo FAPESP: 19/17433-3 - Avaliação dos efeitos citotóxicos ou neuroprotetores de peptídeos alterados no sistema nervoso central de zebrafish (Danio rerio) na Doença de Parkinson
• Beneficiário: Louise Oliveira Fiametti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/16023-6 - Revelando dados de transcriptoma e proteômica para caracterização de peptidomas e peptídeos bioativos em organismos da fauna acompanhante
• Beneficiário: Leandro Mantovani de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Regular