Peptide Profile of Zebrafish Brain in a 6-OHDA-Induced Parkinson Model

Author(s)

Fiametti, Louise Oliveira ^[1] ; Correa, Claudia Neves ^{[1, 2]} ; Castro, Leandro Mantovani de ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Biosci Inst, BR-11330900 Sao Vicente - Brazil

^[2] Sao Paulo State Univ UNESP, Biosci Inst, Biodivers Coastal Environm Postgrad Program, Sao Vicente - Brazil

• Document type: Journal article
• Source: ZEBRAFISH; v. 18, n. 1 FEB 2021.
• Web of Science Citations: 0

Abstract

Parkinson's disease (PD) is a chronic neurodegenerative disorder mainly attributed to the progressive loss of dopaminergic neurons in the substantia nigra, which leads to uncontrolled voluntary movements causing tremors, postural instability, joint stiffness, and speech and locomotion difficulties, among other symptoms. Previous studies have shown the participation of specific peptides in neurodegenerative diseases. In this context, the present work analyzed changes in the peptide profile in zebrafish brain induced to parkinsonian conditions with 6-hydroxydopamine, using isotopic labeling techniques plus mass spectrometry. These analyses allowed the relative quantitation and identification of 118 peptides. Of these, nine peptides showed significant changes, one peptide was increased and eight decreased. The most altered sequences were fragment of cytosolic and extracellular proteins related to lipid metabolism and dynamic cytoskeleton. These results open new perspectives of study about the function of peptides in PD. (AU)

• FAPESP's process: 19/17433-3 - Evaluation of cytotoxic or neuroprotective effects of altered peptides on the central nervous system of zebrafish (Danio rerio) in Parkinson's Disease
• Grantee: Louise Oliveira Fiametti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/16023-6 - Revealing transcriptome and proteome data for characterization of peptidomes and bioactive peptides in organisms from bycatch of shrimp trawling
• Grantee: Leandro Mantovani de Castro
• Support Opportunities: Regular Research Grants