Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11

Autor(es)

Gelain, Lucas ^{[1, 2]} ; Kingma, Esther ^[1] ; da Cruz Pradella, Jose Geraldo ^[3] ; da Costa, Aline Carvalho ^[2] ; van der Wielen, Luuk ^{[4, 1]} ; van Gulik, Walter M. ^[1]

Afiliação do(s) autor(es)

^[1] Delft Univ Technol, Dept Biotechnol, Van Maasweg 9, NL-2629 HZ Delft - Netherlands

^[2] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, Campinas - Brazil

^[3] Univ Fed Sao Paulo, Inst Sci & Technol, Av Cesare Mansueto Giulio Lattes 1201, Sao Jose Dos Campos - Brazil

^[4] Univ Limerick, Bernal Inst, Limerick V94 T9PX - Ireland

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 125, n. 3, p. 177-183, MAR 2021.
• Citações Web of Science: 0

Resumo

Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate of 0.05 h(-1). Experiments using glucose, fructose/glucose and sucrose were performed in duplicate. Glucose condition was found to induce the production of enzymes that can catalyse the hydrolysis of p-nitro-phenyl-beta-D-glucopyranoside (PNPGase). A concentration of 20 g/L of glucose in the feed provided the highest productivity (1048 +/- 16 U/mol h). Extracellular polysaccharides were considered the source of inducers. Based on the obtained results, a new PNPGase production process was developed using mainly glucose. This process raises interesting possibilities of synthesizing the inducer substrate and the induced enzymes in a single step using an easily assimilated carbon source under carbon-limited conditions. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society. (AU)

• Processo FAPESP: 14/22537-9 - Modelagem matemática da produção de enzimas pelo Trichoderma harzianum em fermentação submersa
• Beneficiário: Lucas Gelain
• Modalidade de apoio: Bolsas no Brasil - Doutorado