| Full text | |
| Author(s): |
Gelain, Lucas
[1, 2]
;
Kingma, Esther
[1]
;
da Cruz Pradella, Jose Geraldo
[3]
;
da Costa, Aline Carvalho
[2]
;
van der Wielen, Luuk
[4, 1]
;
van Gulik, Walter M.
[1]
Total Authors: 6
|
| Affiliation: | [1] Delft Univ Technol, Dept Biotechnol, Van Maasweg 9, NL-2629 HZ Delft - Netherlands
[2] Univ Estadual Campinas, Sch Chem Engn, Av Albert Einstein 500, Campinas - Brazil
[3] Univ Fed Sao Paulo, Inst Sci & Technol, Av Cesare Mansueto Giulio Lattes 1201, Sao Jose Dos Campos - Brazil
[4] Univ Limerick, Bernal Inst, Limerick V94 T9PX - Ireland
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FUNGAL BIOLOGY; v. 125, n. 3, p. 177-183, MAR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate of 0.05 h(-1). Experiments using glucose, fructose/glucose and sucrose were performed in duplicate. Glucose condition was found to induce the production of enzymes that can catalyse the hydrolysis of p-nitro-phenyl-beta-D-glucopyranoside (PNPGase). A concentration of 20 g/L of glucose in the feed provided the highest productivity (1048 +/- 16 U/mol h). Extracellular polysaccharides were considered the source of inducers. Based on the obtained results, a new PNPGase production process was developed using mainly glucose. This process raises interesting possibilities of synthesizing the inducer substrate and the induced enzymes in a single step using an easily assimilated carbon source under carbon-limited conditions. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society. (AU) | |
| FAPESP's process: | 14/22537-9 - Mathematical modeling of enzymes production by Trichoderma harzianum in submerged fermentation |
| Grantee: | Lucas Gelain |
| Support Opportunities: | Scholarships in Brazil - Doctorate |