Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging

Autor(es):Mostrar menos -

Raja, Sebastian ^{[1, 2]} ; Hamouda, I, Ahmed E. ; de Toledo, Marcelo A. S. ^[3] ; Hu, Chaolei ^[4] ; Bernardo, Marcela P. ^{[1, 3]} ; Schalla, Carmen ^[3] ; Leite, Liliane S. F. ^[1] ; Buhl, Eva Miriam ^[5] ; Dreschers, Stephan ^[6] ; Pich, Andrij ^[4] ; Zenke, Martin ^[3] ; Mattoso, Luiz H. C. ^[1] ; Sechi, Antonio ^[3]

Afiliação do(s) autor(es)

^[1] EMBRAPA Instrumentacao, Natl Nanotechnol Lab Agribusiness LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[3] Hamouda, Ahmed E., I, Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[4] Rhein Westfal TH Aachen, DWI Leibniz Inst Interact Mat, D-52074 Aachen - Germany

^[5] Rhein Westfal TH Aachen, Inst Pathol, Electron Microscopy Facil, D-52074 Aachen - Germany

^[6] Rhein Westfal TH Aachen, Klin Kinder & Jugendmed, D-52074 Aachen - Germany

• Tipo de documento: Artigo Científico
• Fonte: Biomacromolecules; v. 22, n. 2, p. 454-466, FEB 2021.
• Citações Web of Science: 0

Resumo

Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells. (AU)

• Processo FAPESP: 15/00094-0 - Modificações químicas de nanowhiskers de celulose
• Beneficiário: Raja Sebastian
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/22017-3 - Nanocristais de celulose (NIR - CNC) de infravermelho próximo (NIR) para bioimagem celular
• Beneficiário: Raja Sebastian
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/03080-3 - Preparação e caracterização de bionanocompósitos a base de proteínas e nanocristais de celulose por casting contínuo
• Beneficiário: Liliane Samara Ferreira Leite
• Modalidade de apoio: Bolsas no Brasil - Doutorado