Chromosomal Polymorphism and Speciation: The Case of the Genus Mazama (Cetartiodactyla; Cervidae)

Autor(es)

Galindo, David Javier ^[1] ; Martins, Gabriela Siqueira ^[2] ; Vozdova, Miluse ^[3] ; Cernohorska, Halina ^[3] ; Kubickova, Svatava ^[3] ; Bernegossi, Agda Maria ^[1] ; Kadlcikova, Dita ^[3] ; Rubes, Jiri ^[3] ; Duarte, Jose Mauricio Barbanti ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Nucleo Pesquisa & Conservacao Cervideos, NUPECCE, UNESP, BR-14884900 Jaboticabal - Brazil

^[2] Univ Sao Paulo, Lab Dosagens Hormonais, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil

^[3] Cent European Inst Technol, Vet Res Inst, Dept Genet & Reprod Biotechnol, Brno 62100 - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 12, n. 2 FEB 2021.
• Citações Web of Science: 0

Resumo

Chromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus Mazama that comprises wide inter- and intra-specific chromosomal variability. To evaluate the impact of chromosomal polymorphisms as reproductive barriers within the genus Mazama, inter-specific hybrids between Mazama gouazoubira and Mazama nemorivaga (MGO x MNE) and intra-specific hybrids between cytotypes of Mazama americana (MAM) differing by a tandem (TF) or centric fusion (Robertsonian translocations-RT) were evaluated. MGO x MNE hybrid fertility was evaluated by the seminal quality and testicular histology. MAM hybrids estimation of the meiotic segregation products was performed by sperm-FISH analysis. MGO x MNE hybrids analyses showed different degrees of fertility reduction, from severe subfertility to complete sterility. Regarding MAM, RT, and TF carriers showed a mean value for alternate segregation rate of 97.74%, and 67.23%, and adjacent segregation rate of 1.80%, and 29.07%, respectively. Our results suggested an efficient post-zygotic barrier represented by severe fertility reduction for MGO x MNE and MAM with heterozygous TF. Nevertheless, RT did not show a severe effect on the reproductive fitness in MAM. Our data support the validity of MGO and MNE as different species and reveals cryptic species within MAM. (AU)

• Processo FAPESP: 17/07014-8 - O uso de topotipos atuais para produção de genótipos e citótipos na revisão taxonômica do gênero Mazama: a base para a conservação das espécies
• Beneficiário: José Maurício Barbanti Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/50748-3 - Os veados cinza do Brasil (Mammalia: Cervidae: Mazama): a busca das variantes genéticas, morfológicas e ecológicas no sentido de explicar a complexa taxonomia e evolução do grupo
• Beneficiário: José Maurício Barbanti Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/06940-1 - O uso da citogenética molecular para estudos evolutivos e taxonômicos em cervídeos
• Beneficiário: José Maurício Barbanti Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular