| Full text | |
| Author(s): |
Galindo, David Javier
[1]
;
Martins, Gabriela Siqueira
[2]
;
Vozdova, Miluse
[3]
;
Cernohorska, Halina
[3]
;
Kubickova, Svatava
[3]
;
Bernegossi, Agda Maria
[1]
;
Kadlcikova, Dita
[3]
;
Rubes, Jiri
[3]
;
Duarte, Jose Mauricio Barbanti
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Nucleo Pesquisa & Conservacao Cervideos, NUPECCE, UNESP, BR-14884900 Jaboticabal - Brazil
[2] Univ Sao Paulo, Lab Dosagens Hormonais, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil
[3] Cent European Inst Technol, Vet Res Inst, Dept Genet & Reprod Biotechnol, Brno 62100 - Czech Republic
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | GENES; v. 12, n. 2 FEB 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Chromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus Mazama that comprises wide inter- and intra-specific chromosomal variability. To evaluate the impact of chromosomal polymorphisms as reproductive barriers within the genus Mazama, inter-specific hybrids between Mazama gouazoubira and Mazama nemorivaga (MGO x MNE) and intra-specific hybrids between cytotypes of Mazama americana (MAM) differing by a tandem (TF) or centric fusion (Robertsonian translocations-RT) were evaluated. MGO x MNE hybrid fertility was evaluated by the seminal quality and testicular histology. MAM hybrids estimation of the meiotic segregation products was performed by sperm-FISH analysis. MGO x MNE hybrids analyses showed different degrees of fertility reduction, from severe subfertility to complete sterility. Regarding MAM, RT, and TF carriers showed a mean value for alternate segregation rate of 97.74%, and 67.23%, and adjacent segregation rate of 1.80%, and 29.07%, respectively. Our results suggested an efficient post-zygotic barrier represented by severe fertility reduction for MGO x MNE and MAM with heterozygous TF. Nevertheless, RT did not show a severe effect on the reproductive fitness in MAM. Our data support the validity of MGO and MNE as different species and reveals cryptic species within MAM. (AU) | |
| FAPESP's process: | 17/07014-8 - The use of actual topotypes to produce genotypes and cytotypes in the taxonomic review of the Mazama genus: the basis for the species conservation |
| Grantee: | José Maurício Barbanti Duarte |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 10/50748-3 - The gray brockets in Brazil (Mammalia; Cervidae; Mazama): detection of the genetic, morphologic and ecologic variants to explain the complex evolution in this group |
| Grantee: | José Maurício Barbanti Duarte |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/06940-1 - The use of molecular cytogenetics for evolutioonary and taxonomic studies in Cervidae |
| Grantee: | José Maurício Barbanti Duarte |
| Support Opportunities: | Regular Research Grants |