A two-step resin based approach to reveal survivin-selective fluorescent probes

Autor(es):Mostrar menos -

Ambrose, Andrew J. ^[1] ; Pham, Nhan T. ^{[2, 3]} ; Sivinski, Jared ^[1] ; Guimaraes, Larissa ^{[4, 1, 5]} ; Mollasalehi, Niloufar ^[1] ; Jimenez, Paula ^[6] ; Abad, Maria A. ^[7] ; Jeyaprakash, A. Arockia ^[7] ; Shave, Steven ^{[2, 3]} ; Costa-Lotufo, Leticia V. ^[4] ; La Clair, James J. ^[8] ; Auer, Manfred ^{[2, 3]} ; Chapman, Eli ^[1]

Afiliação do(s) autor(es)

^[1] Univ Arizona, Coll Pharm, Dept Pharmacol & Toxicol, Tucson, AZ 85721 - USA

^[2] Univ Edinburgh, Sch Biol Sci, Kings Bldg, CH Waddington Bldg 3-07, Edinburgh EH9 3BF, Midlothian - Scotland

^[3] Univ Edinburgh, Edinburgh Med Sch, Biomed Sci, Kings Bldg, CH Waddington Bldg 3-07, Edinburgh EH9 3BF, Midlothian - Scotland

^[4] Univ Sao Paulo, Dept Farmacol, BR-05508900 Sao Paulo, SP - Brazil

^[5] Univ Fed Piaui, Campus Senador Helvidio Nunes de Barros, BR-64607670 Picos, PI - Brazil

^[6] Univ Fed Sao Paulo, Inst Mar, BR-11070100 Santos, SP - Brazil

^[7] Univ Edinburgh, Wellcome Ctr Cell Biol, Edinburgh EH9 3BF, Midlothian - Scotland

^[8] Xenobe Res Inst, POB 3052, San Diego, CA 92163 - USA

• Tipo de documento: Artigo Científico
• Fonte: RSC CHEMICAL BIOLOGY; v. 2, n. 1, p. 181-186, FEB 1 2021.
• Citações Web of Science: 2

Resumo

The identification of modulators for proteins without assayable biochemical activity remains a challenge in chemical biology. The presented approach adapts a high-throughput fluorescence binding assay and functional chromatography, two protein-resin technologies, enabling the discovery and isolation of fluorescent natural product probes that target proteins independently of biochemical function. The resulting probes also suggest targetable pockets for lead discovery. Using human survivin as a model, we demonstrate this method with the discovery of members of the prodiginine family as fluorescent probes to the cancer target survivin. (AU)

• Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático