Taurine treatment reverses protein malnutrition-induced endothelial dysfunction of the pancreatic vasculature: The role of hydrogen sulfide

Autor(es)

Guizoni, Daniele M. ^[1] ; Freitas, Israelle N. ^[1] ; Victorio, Jamaira A. ^[1] ; Possebom, Isabela R. ^[1] ; Araujo, Thiago R. ^[2] ; Carneiro, Everardo M. ^[2] ; Davel, Ana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Struct & Funct Biol, Inst Biol, Lab Vasc Biol, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Struct & Funct Biol, Inst Biol, Obes & Comorbid Res Ctr OCRC, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 116, MAR 2021.
• Citações Web of Science: 1

Resumo

Background: Protein malnutrition in childhood predisposes individuals to vascular and pancreatic endocrine dysfunction, thus increasing the risk of diabetes and hypertension. Because taurine may reduce cardiometabolic risk, we hypothesized that taurine treatment has a beneficial effect on the pancreatic vasculature during protein restriction. Methods and results: Weaned mice were fed a normal or a low-protein diet and were treated with or without taurine for 3 months. The lieno-pancreatic artery (LPA) from low-protein diet-treated mice exhibited impaired endothelium dependent relaxation to acetylcholine that was associated with decreased endothelium-derived hyperpolarization (EDH), hydrogen sulfide (H2S) production, and H2S-synthesizing CBS expression and impaired vasorelaxation to an H2S-donor, NaHS. These changes were prevented by taurine treatment. We compared the effects of taurine with the effects of the direct vasodilator hydralazine and found that both normalized blood pressure and the endothelial vasodilator function of the LPA in the mice fed a protein-restricted diet. However, only taurine restored the CBS expression in the LPA and insulin secretion in response to high glucose. The LPA supplies the pancreas and shares morphometry with the mesenteric resistance artery (MRA). However, in the MRA, low-protein diet-induced endothelial dysfunction is driven by impaired NOS-derived NO with no changes in H2S signaling. Conclusions: The results suggest that taurine protects against protein malnutrition-induced endothelial dysfunction in the LPA by upregulating the CBS-H2S pathway. Considering the importance of the pancreatic vasculature for endocrine islet activity, taurine may be a potential therapy for the vascular and metabolic dysfunction associated with malnutrition and comorbidities. (C) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/01717-9 - Investigação dos efeitos insulinotrópicos, insulinomiméticos e endoteliais da taurina em células/tecidos submetidos à restrição in vitro de aminoácidos: uma abordagem integrada e multifocal
• Beneficiário: Everardo Magalhães Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/14461-8 - Investigação dos efeitos endoteliais da taurina na restrição protéica: uma abordagem in vivo e in vitro
• Beneficiário: Daniele Mendes Guizoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado