Adaptive epigenetic response of glutathione (GSH)-related genes against lead (Pb)-induced toxicity, in individuals chronically exposed to the metal

Autor(es):Mostrar menos -

Devoz, Paula Picoli ^[1] ; dos Reis, Mariana Bisarro ^[2] ; Gomes, Willian Robert ^[1] ; Maraslis, Flora Troina ^[3] ; Ribeiro, Diego Luis ^[4] ; Greggi Antunes, Lusania Maria ^[1] ; Batista, Bruno Lemos ^[5] ; Grotto, Denise ^[6] ; Reis, Rui Manuel ^{[2, 7, 8]} ; Barbosa Jr, Fernando ; Mazzaron Barcelos, Gustavo Rafael ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Villela 1331, BR-14784400 Barretos, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Ave Ana Costa 95, 3 Andar, BR-11060001 Santos, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Fed Univ ABC, Ctr Nat & Human Sci, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[6] Univ Sorocaba, Rodovia Raposo Tavares Km 92-5, BR-18023000 Sorocaba, SP - Brazil

^[7] Univ Minho, Sch Med, Life & Hlth Sci Res Inst, Gualtar Campus, P-4710057 Braga - Portugal

^[8] ICVS 3Bs PT Govt Associate Lab, Gualtar Campus, P-4710057 Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 269, APR 2021.
• Citações Web of Science: 0

Resumo

It is well known that one of the most outstanding adverse effects related to lead (Pb) exposure is oxidative stress; moreover, recent findings suggest that disturbances of the redox status of cells are associated with epigenetic responses, and metabolism of glutathione (GSH) plays an important role in this process. This study aimed to assess Pb exposure on % methylation of GSH-related genes' promoter regions (%CH3-CpG) and their influence on biomarkers of oxidative stress, in workers exposed to the metal. One hundred nine male workers participated in the study; ICP-MS determined blood lead levels (BLL); biochemical parameters related to redox status, named GSH, glutathione peroxidase (GPX) and glutathione-S-transferase (GST) were quantified by UV/Vis spectrophotometry. Determination of %CH3-CpG of genes GCLC, GPX1, GSR, and GSTP1 were done by pyrosequencing. Inverse associations were seen between BLL and %CH3-CpG-GCLC, and %CH3-CpG-GSTP1. Moreover, metal exposure did not impact GSH, GPX, and GST; however, negative associations were observed between %CH3-CpG-GPX1 and %CH3-CpG-GSTP1, and the activities of GPX and GST, respectively. Taken together, our results give further evidence about adaptive epigenetic response to avoid oxidative damage induced by Pb exposure, allowing a better understanding of the molecular mechanisms related to the metal toxicity. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/24981-3 - Integração de ferramentas ômicas para o estudo de modelos multivariados nas interações gene-ambiente em população expostas a metais tóxicos
• Beneficiário: Gustavo Rafael Mazzaron Barcelos
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 13/06033-8 - Integração de ferramentas ômicas para o estudo de modelos multivariados nas interações gene-ambiente em população expostas a metais tóxicos
• Beneficiário: Gustavo Rafael Mazzaron Barcelos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/07277-3 - Avaliação da exposição ao chumbo sobre o padrão de metilação de regiões promotoras de genes relacionados ao metabolismo do metal, em trabalhadores de fábricas de baterias automotivas
• Beneficiário: Paula Pícoli Devóz
• Modalidade de apoio: Bolsas no Brasil - Mestrado