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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adaptive epigenetic response of glutathione (GSH)-related genes against lead (Pb)-induced toxicity, in individuals chronically exposed to the metal

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Devoz, Paula Picoli [1] ; dos Reis, Mariana Bisarro [2] ; Gomes, Willian Robert [1] ; Maraslis, Flora Troina [3] ; Ribeiro, Diego Luis [4] ; Greggi Antunes, Lusania Maria [1] ; Batista, Bruno Lemos [5] ; Grotto, Denise [6] ; Reis, Rui Manuel [2, 7, 8] ; Barbosa Jr, Fernando ; Mazzaron Barcelos, Gustavo Rafael [3]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Villela 1331, BR-14784400 Barretos, SP - Brazil
[3] Univ Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Ave Ana Costa 95, 3 Andar, BR-11060001 Santos, SP - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[5] Fed Univ ABC, Ctr Nat & Human Sci, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil
[6] Univ Sorocaba, Rodovia Raposo Tavares Km 92-5, BR-18023000 Sorocaba, SP - Brazil
[7] Univ Minho, Sch Med, Life & Hlth Sci Res Inst, Gualtar Campus, P-4710057 Braga - Portugal
[8] ICVS 3Bs PT Govt Associate Lab, Gualtar Campus, P-4710057 Braga - Portugal
Total Affiliations: 8
Document type: Journal article
Source: Chemosphere; v. 269, APR 2021.
Web of Science Citations: 0
Abstract

It is well known that one of the most outstanding adverse effects related to lead (Pb) exposure is oxidative stress; moreover, recent findings suggest that disturbances of the redox status of cells are associated with epigenetic responses, and metabolism of glutathione (GSH) plays an important role in this process. This study aimed to assess Pb exposure on % methylation of GSH-related genes' promoter regions (%CH3-CpG) and their influence on biomarkers of oxidative stress, in workers exposed to the metal. One hundred nine male workers participated in the study; ICP-MS determined blood lead levels (BLL); biochemical parameters related to redox status, named GSH, glutathione peroxidase (GPX) and glutathione-S-transferase (GST) were quantified by UV/Vis spectrophotometry. Determination of %CH3-CpG of genes GCLC, GPX1, GSR, and GSTP1 were done by pyrosequencing. Inverse associations were seen between BLL and %CH3-CpG-GCLC, and %CH3-CpG-GSTP1. Moreover, metal exposure did not impact GSH, GPX, and GST; however, negative associations were observed between %CH3-CpG-GPX1 and %CH3-CpG-GSTP1, and the activities of GPX and GST, respectively. Taken together, our results give further evidence about adaptive epigenetic response to avoid oxidative damage induced by Pb exposure, allowing a better understanding of the molecular mechanisms related to the metal toxicity. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/24981-3 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals
Grantee:Gustavo Rafael Mazzaron Barcelos
Support Opportunities: Scholarships in Brazil - Young Researchers
FAPESP's process: 13/06033-8 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals
Grantee:Gustavo Rafael Mazzaron Barcelos
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/07277-3 - Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries
Grantee:Paula Pícoli Devóz
Support Opportunities: Scholarships in Brazil - Master