Combining omics tools for predicting multivariate models in populations exposed to toxic metals

• Grant number: 13/06033-8
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: January 01, 2015
• End date: December 31, 2018
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Gustavo Rafael Mazzaron Barcelos
• Grantee: Gustavo Rafael Mazzaron Barcelos
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated researchers: Siegfried Knasmueller
• Associated research grant(s): 21/06695-7 - Integrating biomonitoring and omics approaches for predicting adverse health effects induced by methylmercury, in Amazonian riverside communities exposed to the metal, via diet, AP.BTA.JP2
• Associated scholarship(s): 18/16508-7 - Exposure to lead (Pb) in workers of automotive battery factories: combined evaluation using biomarkers of genomic instability, BP.PD; 16/24520-1 - Impact of exposure to lead (Pb) on the expression profile of genes related to metal metabolism in occupationally exposed individuals, BP.DR; 15/07277-3 - Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries, BP.MS; + associated scholarships 15/04786-4 - Evaluation of lead-induced oxidative stress exposure in workers of automotive battery plants, BP.IC; 15/01157-6 - Influence of glutamyl-cysteine ligase (catalytic subunit, GCLC, and modifier, GLCM) gene polymorphisms evaluation on lead concentrations in workers exposed to metal, BP.IC; 14/24981-3 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals, BP.JP - associated scholarships

Abstract

There is a wide variation in concentrations of the biomarkers of exposure, effect, and susceptibility associated with exposure to toxic metals despite similar levels of exposure and this is hypothetically related gene modulation in the process kinetics and dynamics of these metals. However, very little is known about the genome effects on the metabolism and toxicity induced by exposure to lead (Pb) and its various interfaces. Thus, this project aims to assess the genome-metal in individuals exposed to metal. Thus, metal concentrations will be determined in blood, urine and plasma by mass spectrometry (ICP- MS) and several determinations and biochemical parameters related to the toxicity of the metal will also be conducted. Evaluation of polymorphisms, gene expression and methylation patterns of promoter regions of genes associated with toxicokinetics and toxicodynamics of the metal will be conducted to evaluate the gene modulation on the concentrations and toxicity induced by metal, through studies of multivariate models. Thus, it is expected that the obtained results will assist for a better understanding of the interactions between the metal and these genetic variants, an incipient research in the country, but very promising named "genome-environment interactions." (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEVOZ, PAULA PICOLI; GOMES, WILLIAN ROBERT; DE ARAUJO, MARILIA LADEIRA; RIBEIRO, DIEGO LUIS; PEDRON, TATIANA; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 19-21, SI, p. 1098-1105, 2017. (15/04786-4, 14/24981-3, 13/06033-8, 15/07277-3)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; CANO LUIZ, BRUNO LIMONTI; GROTTO, DENISE; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, n. B, SI, p. 42-46, DEC 2018. (13/06033-8, 14/24981-3, 15/07277-3, 15/04786-4, 15/01157-6)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; ARAUJO, MARILIA LADEIRA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Milk and Dairy Products Intake Is Associated with Low Levels of Lead (Pb) in Workers highly Exposed to the Metal. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 178, n. 1, p. 29-35, JUL 2017. (15/04786-4, 14/24981-3, 13/06033-8, 15/07277-3)

MARQUES FERNANDES, KELLY CHRISTINE; MARTINS, JR., AIRTON CUNHA; SOARES DE OLIVEIRA, ANDREIA AVILA; GREGGI ANTUNES, LUSANIA MARIA; DE SYLLOS COLUS, ILCE MARA; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphism of Metallothionein 2A Modifies Lead Body Burden in Workers Chronically Exposed to the Metal. PUBLIC HEALTH GENOMICS, v. 19, n. 1, p. 47-52, 2016. (13/06033-8, 13/17288-7)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; CANO LUIZ, BRUNO LIMONTI; GROTTO, DENISE; BATISTA, BRUNO LEMOS; BARBOSA, FERNANDO, JR.; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, p. 5-pg., 2018-12-01. (15/07277-3, 15/01157-6, 14/24981-3, 13/06033-8, 15/04786-4)

DEVOZ, PAULA PICOLI; DOS REIS, MARIANA BISARRO; GOMES, WILLIAN ROBERT; MARASLIS, FLORA TROINA; RIBEIRO, DIEGO LUIS; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; GROTTO, DENISE; REIS, RUI MANUEL; BARBOSA JR, FERNANDO; et al. Adaptive epigenetic response of glutathione (GSH)-related genes against lead (Pb)-induced toxicity, in individuals chronically exposed to the metal. Chemosphere, v. 269, APR 2021. (14/24981-3, 13/06033-8, 15/07277-3)

DEVOZ, PAULA PICOLI; GOMES, WILLIAN ROBERT; DE ARAUJO, MARILIA LADEIRA; RIBEIRO, DIEGO LUIS; PEDRON, TATIANA; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; BARBOSA, FERNANDO, JR.; MAZZARON BARCELOS, GUSTAVO RAFAEL. Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 19-21, p. 8-pg., 2017-01-01. (13/06033-8, 15/07277-3, 15/04786-4, 14/24981-3)