Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries

• Grant number: 15/07277-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2015
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Gustavo Rafael Mazzaron Barcelos
• Grantee: Paula Pícoli Devóz
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/06033-8 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals, AP.JP

Abstract

Lead is a toxic metal that accumulates in the body and cause several adverse effects, affecting multiple systems. With the goal of reducing occupational poisoning, the Brazilian law determined that the maximum concentration of Pb in blood (Pb-b) workers is 60 ¼g/dL. Little is known about the genetic effects on metabolism and toxicity induced by lead exposure (Pb). Thus, the aim of this project is to assist in a better understanding of the interactions between Pb and epigenetic effects on automotive battery factory workers exposed to the metal. It will be selected 125 workers and after will be determined concentrations of Pb-s and Pb on plasma (Pb-p) by mass spectrometry with inductively coupled plasma (ICP-MS). DNA extraction will be performed and, then, the evaluation of chronic exposure to the metal on the methylation pattern of genes promoter regions GSS (Gene ID 2937), GSR (Gene ID 2936), GPx1 (Gene ID 2876), VDR (Gene ID 7421), GSTP1 (Gene ID 2950), GGT1 (Gene ID 2678), GCLC (Gene ID 2729), GCLM (Gene ID 2730), SLC11a2 (Gene ID 4891), ALAD (Gene ID 2010) e MT2a (Gene ID 4502) will be assessed by pyrosequencing. Thus, it is expected that the results will assist in better understanding of the interactions between the metal and the methylation patterns of genes promoter regions.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; CANO LUIZ, BRUNO LIMONTI; GROTTO, DENISE; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, n. B, SI, p. 42-46, DEC 2018. (13/06033-8, 14/24981-3, 15/07277-3, 15/04786-4, 15/01157-6)

DEVOZ, PAULA PICOLI; DOS REIS, MARIANA BISARRO; GOMES, WILLIAN ROBERT; MARASLIS, FLORA TROINA; RIBEIRO, DIEGO LUIS; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; GROTTO, DENISE; REIS, RUI MANUEL; BARBOSA JR, FERNANDO; et al. Adaptive epigenetic response of glutathione (GSH)-related genes against lead (Pb)-induced toxicity, in individuals chronically exposed to the metal. Chemosphere, v. 269, APR 2021. (14/24981-3, 13/06033-8, 15/07277-3)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; ARAUJO, MARILIA LADEIRA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Milk and Dairy Products Intake Is Associated with Low Levels of Lead (Pb) in Workers highly Exposed to the Metal. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 178, n. 1, p. 29-35, JUL 2017. (15/04786-4, 14/24981-3, 13/06033-8, 15/07277-3)

DEVOZ, PAULA PICOLI; GOMES, WILLIAN ROBERT; DE ARAUJO, MARILIA LADEIRA; RIBEIRO, DIEGO LUIS; PEDRON, TATIANA; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; BARBOSA, FERNANDO, JR.; MAZZARON BARCELOS, GUSTAVO RAFAEL. Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 19-21, p. 8-pg., 2017-01-01. (13/06033-8, 15/07277-3, 15/04786-4, 14/24981-3)

GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; CANO LUIZ, BRUNO LIMONTI; GROTTO, DENISE; BATISTA, BRUNO LEMOS; BARBOSA, FERNANDO, JR.; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, p. 5-pg., 2018-12-01. (15/07277-3, 15/01157-6, 14/24981-3, 13/06033-8, 15/04786-4)

DEVOZ, PAULA PICOLI; GOMES, WILLIAN ROBERT; DE ARAUJO, MARILIA LADEIRA; RIBEIRO, DIEGO LUIS; PEDRON, TATIANA; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 19-21, SI, p. 1098-1105, 2017. (15/04786-4, 14/24981-3, 13/06033-8, 15/07277-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DEVÓZ, Paula Pícoli. Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.