Lead is a toxic metal that accumulates in the body and cause several adverse effects, affecting multiple systems. With the goal of reducing occupational poisoning, the Brazilian law determined that the maximum concentration of Pb in blood (Pb-b) workers is 60 ¼g/dL. Little is known about the genetic effects on metabolism and toxicity induced by lead exposure (Pb). Thus, the aim of this project is to assist in a better understanding of the interactions between Pb and epigenetic effects on automotive battery factory workers exposed to the metal. It will be selected 125 workers and after will be determined concentrations of Pb-s and Pb on plasma (Pb-p) by mass spectrometry with inductively coupled plasma (ICP-MS). DNA extraction will be performed and, then, the evaluation of chronic exposure to the metal on the methylation pattern of genes promoter regions GSS (Gene ID 2937), GSR (Gene ID 2936), GPx1 (Gene ID 2876), VDR (Gene ID 7421), GSTP1 (Gene ID 2950), GGT1 (Gene ID 2678), GCLC (Gene ID 2729), GCLM (Gene ID 2730), SLC11a2 (Gene ID 4891), ALAD (Gene ID 2010) e MT2a (Gene ID 4502) will be assessed by pyrosequencing. Thus, it is expected that the results will assist in better understanding of the interactions between the metal and the methylation patterns of genes promoter regions.
News published in Agência FAPESP Newsletter about the scholarship: