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Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries

Grant number: 15/07277-3
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2015
Effective date (End): July 31, 2017
Field of knowledge:Biological Sciences - Pharmacology - Toxicology
Principal researcher:Gustavo Rafael Mazzaron Barcelos
Grantee:Paula Pícoli Devóz
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:13/06033-8 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals, AP.JP

Abstract

Lead is a toxic metal that accumulates in the body and cause several adverse effects, affecting multiple systems. With the goal of reducing occupational poisoning, the Brazilian law determined that the maximum concentration of Pb in blood (Pb-b) workers is 60 ¼g/dL. Little is known about the genetic effects on metabolism and toxicity induced by lead exposure (Pb). Thus, the aim of this project is to assist in a better understanding of the interactions between Pb and epigenetic effects on automotive battery factory workers exposed to the metal. It will be selected 125 workers and after will be determined concentrations of Pb-s and Pb on plasma (Pb-p) by mass spectrometry with inductively coupled plasma (ICP-MS). DNA extraction will be performed and, then, the evaluation of chronic exposure to the metal on the methylation pattern of genes promoter regions GSS (Gene ID 2937), GSR (Gene ID 2936), GPx1 (Gene ID 2876), VDR (Gene ID 7421), GSTP1 (Gene ID 2950), GGT1 (Gene ID 2678), GCLC (Gene ID 2729), GCLM (Gene ID 2730), SLC11a2 (Gene ID 4891), ALAD (Gene ID 2010) e MT2a (Gene ID 4502) will be assessed by pyrosequencing. Thus, it is expected that the results will assist in better understanding of the interactions between the metal and the methylation patterns of genes promoter regions.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; CANO LUIZ, BRUNO LIMONTI; GROTTO, DENISE; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, n. B, SI, p. 42-46, DEC 2018. Web of Science Citations: 1.
GOMES, WILLIAN ROBERT; DEVOZ, PAULA PICOLI; ARAUJO, MARILIA LADEIRA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Milk and Dairy Products Intake Is Associated with Low Levels of Lead (Pb) in Workers highly Exposed to the Metal. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 178, n. 1, p. 29-35, JUL 2017. Web of Science Citations: 3.
DEVOZ, PAULA PICOLI; GOMES, WILLIAN ROBERT; DE ARAUJO, MARILIA LADEIRA; RIBEIRO, DIEGO LUIS; PEDRON, TATIANA; GREGGI ANTUNES, LUSANIA MARIA; BATISTA, BRUNO LEMOS; BARBOSA, JR., FERNANDO; MAZZARON BARCELOS, GUSTAVO RAFAEL. Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 19-21, SI, p. 1098-1105, 2017. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DEVÓZ, Paula Pícoli. Evaluation of lead exposure on the methylation pattern of promoter regions of genes associated to the metal metabolism, in workers of automotive battery industries. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.