| Grant number: | 11/22575-0 |
| Support type: | Scholarships abroad - Research Internship - Post-doctor |
| Effective date (Start): | June 01, 2012 |
| Effective date (End): | September 30, 2012 |
| Field of knowledge: | Health Sciences - Pharmacy |
| Principal Investigator: | Fernando Barbosa Júnior |
| Grantee: | Gustavo Rafael Mazzaron Barcelos |
| Supervisor abroad: | Karin Broberg |
| Home Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Local de pesquisa : | Lund University, Lund, Sweden |
| Associated to the scholarship: | 10/16691-4 - Evaluation of mercury and lead metabolism and toxicity related polymorphisms in riverside communities exposed to the metals, in Amazonian region, BP.PD |
Abstract Mercury (Hg) is one of the most toxic metals in the environment, causing many adverse health effects. Its elimination is done by slowly conjunction with glutathione (GSH) through a detoxification system that involves several steps with this compound. Thus, the hypothesis being examined in this project is that the genetic polymorphism related to the enzyme-glutamyl-cysteine-ligase M (GCLM-588), which participates in the synthesis of GSH, may influence the process of elimination of methylmercury (MeHg) in individuals environmentally exposed to this metal. For that, we intend to perform genotyping of the gene in riparian communities chronically exposed to MeHg through consumption of contaminated fish in the Tapajós River region, Pará, using the method for detecting polymorphisms TaqMan®, by use of Real Time Polymerase Chain Reaction (RT-PCR) and correlate with the concentrations of total Hg in blood, plasma, hair, as well as its relations blood/hair and blood/plasma (results already obtained) and to evaluate whether this polymorphism influences the concentrations of the metal in such biomarkers. In addition, this study will also focuses on the previously obtained data (Pos-doc project FAPESP 2010/16691-4) polymorphisms of the genes of glutathione S-transferases GSTM1, GSTT1 and GSTP1 and epidemiological data of the population and evaluate their possible influences and correlations using multivariate linear analysis. Therefore, the results obtained in this study will bring relevant information on environmental exposure to MeHg and its consequences to human health, the possible influence of polymorphisms of genes related to detoxification system mediated by GSH on the concentrations of this metal in individuals and even be shown various toxicological effects between genetic variations in the population and exposure to MeHg. (AU) | |