| Texto completo | |
| Autor(es): |
Bourgard, Catarina
[1]
;
Lopes, Stefanie C. P.
[2, 3]
;
Lacerda, Marcus V. G.
[2, 3]
;
Albrecht, Letusa
[1, 4]
;
Costa, Fabio T. M.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Lab Trop Dis, Campinas, SP - Brazil
[2] Fundacao Oswaldo Cruz FIOCRUZ, Inst Leonidas & Maria Deane, Manaus, AM - Brazil
[3] Fundacao Med Trop Dr Heitor Vieira Dourado FMT HV, Gerencia Malaria, Manaus, AM - Brazil
[4] Fundacao Oswaldo Cruz Fiocruz, Inst Carlos, Curitiba, PR - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 11, n. 1 MAR 3 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
Plasmodium vivax is a world-threatening human malaria parasite, whose biology remains elusive. The unavailability of in vitro culture, and the difficulties in getting a high number of pure parasites makes RNA isolation in quantity and quality a challenge. Here, a methodological outline for RNA-seq from P. vivax isolates with low parasitemia is presented, combining parasite maturation and enrichment with efficient RNA extraction, yielding similar to 100 pg.mu L-1 of RNA, suitable for SMART-Seq Ultra-Low Input RNA library and Illumina sequencing. Unbiased coding transcriptome of similar to 4 M reads was achieved for four patient isolates with similar to 51% of transcripts mapped to the P. vivax P01 reference genome, presenting heterogeneous profiles of expression among individual isolates. Amongst the most transcribed genes in all isolates, a parasite-staged mixed repertoire of conserved parasite metabolic, membrane and exported proteins was observed. Still, a quarter of transcribed genes remain functionally uncharacterized. In parallel, a P. falciparum Brazilian isolate was also analyzed and 57% of its transcripts mapped against IT genome. Comparison of transcriptomes of the two species revealed a common trophozoite-staged expression profile, with several homologous genes being expressed. Collectively, these results will positively impact vivax research improving knowledge of P. vivax biology. (AU) | |
| Processo FAPESP: | 12/16525-2 - Plasmodium vivax: patogênese e infectividade |
| Beneficiário: | Fabio Trindade Maranhão Costa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/20509-5 - Análise dos mecanismos imunopatológicos e moleculares envolvidos no processo de citoaderência de Plasmodium vivax |
| Beneficiário: | Catarina Baeta da Luz Bourgard |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/18611-7 - Desenvolvimento de novas ferramentas para busca e validação de alvos moleculares para terapia contra Plasmodium vivax |
| Beneficiário: | Fabio Trindade Maranhão Costa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |