The In Vitro Effect of Ozone Therapy Against Equine Pythium insidiosum

Autor(es)

Ferreira, Jair Camargo ^[1] ; Pires, Regina Helena ^[2] ; da Costa, Guilherme Barbosa ^[1] ; Carrijo, Bruna Nonato ^[1] ; Guiotto, Francine Gabriela ^[1] ; Rodrigues, Viviani Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Postgrad Program Anim Sci, Sao Paulo - Brazil

^[2] Univ Franca, Postgrad Program Hlth Promot, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Equine Veterinary Science; v. 98, MAR 2021.
• Citações Web of Science: 0

Resumo

The goal of the present study was to characterize the antimicrobial action of different ozone (O-3) presentations against Pythium insidiosum isolated from horses. In experiment 1, P. insidiosum was treated with ozonated distilled water, ozonated sunflower oils with distinct peroxide indexes or O-3 gas (72 mu g O-3 mL(-1)). In experiment 2, samples were exposed one or three times to oxygen (O-2) or O-3 gas (72 mu g O-3 mL(-1); 30 min/ day). In experiment 3, P. insidiosum was treated with different concentrations of O-3 gas (\& Oslash;, 32, 52, or 72 mu g O-3 mL(-1)) for three days (30 min/day). In experiment 4, samples were exposed to O-2 or O-3 gas (72 mu g O-3 mL(-1)) for 05,15, or 30 minutes during three days. Posteriorly, all samples were cultured for two weeks, and the pathogen growth area was measured until D14. Samples with absence of growth on D14 were recultured to assess the germicidal or germistatic action of the treatment. In experiment 1, only ozonized sunflower oil with a high peroxide index had germicidal action against the pathogen. In experiment 2, samples exposed three times to O-3 gas were inactivated. In experiment 3, the O-3 therapy had germicidal action against P. insidiosum independently of the gas concentration (P > .1). In experiment 4, O-3 treatments >15 minutes suppressed the pathogen development, whereas samples exposed to O-3 gas for 5 minutes had progressive growth (P < .01). In conclusion, ozonated sunflower oil with a high peroxide index and multiple exposures to O-3 gas mixtures were able to inactivate P. insidiosum isolated from horses. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 19/13812-0 - Uso de diferentes apresentações do ozônio na inativação do Pythium insidiosum - estudo in vitro
• Beneficiário: Francine Gabriela Guiotto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/15532-4 - Inativação do Pythium insidiosum com gás rico em ozônio: efeito de dose e tempo de exposição
• Beneficiário: Viviani Silva Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica