Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence

Autor(es)

Andre, Carolina Bosso ^[1] ; Rosalen, Pedro Luiz ^{[2, 3]} ; Giannini, Marcelo ^[4] ; Bueno-Silva, Bruno ^[5] ; Pfeifer, Carmem Silvia ^[6] ; Ferracane, Jack Liborio ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Sch Dent, Dept Restorat Dent, Belo Horizonte, MG - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Biosci, Piracicaba, SP - Brazil

^[3] Univ Fed Alfenas, Biol Sci Grad Program, Alfenas, MG - Brazil

^[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil

^[5] Univ Guarulhos, Sch Dent, Dent Res Div, Guarulhos, SP - Brazil

^[6] Oregon Hlth & Sci Univ, Sch Dent, Dept Biomat & Biomech, Portland, OR 97201 - USA

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 37, n. 4, p. e201-e212, APR 2021.
• Citações Web of Science: 0

Resumo

Objectives. The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods. Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and watersoluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results. The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance. Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites. (c) 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/22823-9 - Influência da incorporação de produtos naturais nas propriedades mecânicas e antimicrobiana de sistemas adesivos
• Beneficiário: Carolina Bosso André
• Modalidade de apoio: Bolsas no Brasil - Doutorado