Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis

Autor(es)

Azuma, Mariane Maffei ^{[1, 2]} ; Morais Cardoso, Carolina de Barros ^[2] ; Samuel, Renata Oliveira ^{[2, 3]} ; Pipa, Camila Barbosa ^[2] ; Mogami Bomfim, Suely Regina ^[4] ; Narciso, Luis Gustavo ^[4] ; Gomes-Filho, Joao Eduardo ^[2] ; Angelo Cintra, Luciano Tavares ^[2]

Afiliação do(s) autor(es)

^[1] Univ Michigan, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 - USA

^[2] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, Sao Paulo - Brazil

^[3] Univ Uberaba, Dent Sch, Dept Clin Dent, Uberaba, MG - Brazil

^[4] Sao Paulo State Univ Unesp, Dept Clin & Surg & Anim Reprod, Aracatuba Vet Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 47, n. 2, p. 272-277, FEB 2021.
• Citações Web of Science: 1

Resumo

Introduction: This study investigated the effects of omega-3 polyunsaturated fatty acids (omega-3 PUFAs) on serum inflammatory mediators of rats with pulp exposure-induced apical periodontitis. Methods: Forty male Wistar rats were divided into the following groups: control, untreated rats (C); control rats treated with omega-3 PUFAs (C-0); rats with pulp exposure-induced apical periodontitis (AP); and rats with pulp exposure-induced apical periodontitis treated with w-3 PUFAs (AP-O). omega-3 PUFAs were administered orally once a day for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed, and then blood and jaw samples were collected. Blood analysis was conducted to determine the total number of leukocytes including neutrophils, monocytes, and lymphocytes. Proinflammatory cytokines tumor necrosis factor alpha, interleukin (IL) 6, and IL-17 were quantified by enzyme-linked immunosorbent assay. Histologic analysis was performed to confirm the development of apical periodontitis. The data were statistically evaluated using analysis of variance and the Tukey posttest. The significance level was set at 5%. Results: The development of apical periodontitis was confirmed in all infected groups. Bone destruction was larger in the AP group compared with the AP-O group (P < .05). Blood analysis showed that the AP and AP-O groups showed higher numbers of lymphocytes, leukocytes, monocytes, eosinophils, and expressions of tumor necrosis factor alpha and IL-6 compared with the C and C-O groups (P < .05). In contrast, the presence of leukocytes, lymphocytes, and the expression of IL-6 decreased in the AP-O group compared with the AP group (P < .05). Conclusions: omega-3 PUFA supplementation influences the systemic effects caused by apical periodontitis, decreasing the number of leukocytes, lymphocytes, and IL-6 in rat blood. (AU)

• Processo FAPESP: 15/03054-0 - Efeito da suplementação alimentar com o ácido graxo ômega 3 nos níveis de triglicérides e na reabsorção óssea de ratos Wistar com infecção endodôntica
• Beneficiário: Carolina de Barros Morais Cardoso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/26390-0 - Influência local e sistêmica da suplementação alimentar com o ácido graxo ômega-3 em ratos com infecção endodôntica
• Beneficiário: Mariane Maffei Azuma
• Modalidade de apoio: Bolsas no Brasil - Doutorado