Multiple clades of Husavirus in South America revealed by next generation sequencing

Autor(es):Mostrar menos -

Foro Ramos, Endrya do Socorro ^[1] ; Rosa, Ulisses Alves ^[1] ; Ribeiro, Geovani de Oliveira ^[1] ; Villanova, Fabiola ^[1] ; de Padua Milagres, Flavio Augusto ^{[2, 3]} ; Brustulin, Rafael ^{[2, 3]} ; Morais, Vanessa dos Santos ^[4] ; Lima Araujo, Emerson Luiz ^[5] ; Pandey, Ramendra Pati ^[6] ; Raj, V. Samuel ^[6] ; Sabino, Ester Cerdeira ^[4] ; Deng, Xutao ^{[7, 8]} ; Delwart, Eric ^{[7, 8]} ; Luchs, Adriana ^[9] ; Leal, Elcio ^[1] ; da Costa, Antonio Charlys ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Para, Inst Ciencias Biol, Lab Divers Viral, Belem, Para - Brazil

^[2] Secretary Hlth Tocantins, Tocantins - Brazil

^[3] Publ Hlth Lab Tocantins State LACEN TO, Tocantins - Brazil

^[4] Univ Sao Paulo, Fac Med, Inst Med Trop, Dept Molestias Infecciosas & Parasitarias, Sao Paulo - Brazil

^[5] Minist Hlth CGLAB DAEVS SVS MS, Dept Hlth Surveillance, Gen Coordinat Publ Hlth Labs Strateg Articulat, Brasilia, DF - Brazil

^[6] SRM Univ, Delhi NCR, Ctr Drug Design Discovery & Dev C4D, Sonepat, Haryana - India

^[7] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA

^[8] Vitalant Res Inst, San Francisco, CA - USA

^[9] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 3 MAR 23 2021.
• Citações Web of Science: 0

Resumo

Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study. (AU)

• Processo FAPESP: 17/00021-9 - Metagenômica viral da Dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/12944-9 - Investigando a evolução de cepas animais de rotavírus infectando humanos
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular