Development of recombinant human granulocyte colony-stimulating factor (nartograstim) production process in Escherichia coli compatible with industrial scale and with no antibiotics in the culture medium

Autor(es)

Eguia, Fara A. P. ^{[1, 2]} ; Mascarelli, Daniele E. ^[1] ; Carvalho, Eneas ^[3] ; Rodriguez, Gretel R. ^[4] ; Makiyama, Edson ^[5] ; Borelli, Primavera ^[5] ; Lieberman, Celia ^[1] ; Ho, Paulo Lee ^[6] ; Barazzone, Giovana C. ^[1] ; Goncalves, Viviane M. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Desenvolvimento Vacinas, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Programa Interunidades Biotecnol, Sao Paulo, SP - Brazil

^[3] Inst Butantan, Lab Bacteriol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Lab Hematol, Fac Ciencias Farmaceut, Sao Paulo, SP - Brazil

^[6] Inst Butantan, Ctr BioInd, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 105, n. 1, p. 169-183, JAN 2021.
• Citações Web of Science: 0

Resumo

The granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine that has important clinical applications for treating neutropenia. Nartograstim is a recombinant variant of human G-CSF. Nartograstim has been produced in Escherichia coli as inclusion bodies (IB) and presents higher stability and biological activity than the wild type of human G-CSF because of its mutations. We developed a production process of nartograstim in a 10-L bioreactor using auto-induction or chemically defined medium. After cell lysis, centrifugation, IB washing, and IB solubilization, the following three refolding methods were evaluated: diafiltration, dialysis, and direct dilution in two refolding buffers. Western blot and SDS-PAGE confirmed the identity of 18.8-kDa bands as nartograstim in both cultures. The auto-induction medium produced 1.17 g/L and chemically defined medium produced 0.95 g/L. The dilution method yielded the highest percentage of refolding (99%). After refolding, many contaminant proteins precipitated during pH adjustment to 5.2, increasing purity from 50 to 78%. After applying the supernatant to cation exchange chromatography (CEC), nartograstim recovery was low and the purity was 87%. However, when the refolding solution was applied to anion exchange chromatography followed by CEC, 91%-98% purity and 2.2% recovery were obtained. The purification process described in this work can be used to obtain nartograstim with high purity, structural integrity, and the expected biological activity.Key points center dot Few papers report the final recovery of the purification process from inclusion bodies.center dot The process developed led to high purity and reasonable recovery compared to literature.center dot Nartograstim biological activity was demonstrated in mice using a neutropenia model. (AU)

• Processo FAPESP: 18/10384-4 - Obtenção e avaliação imunológica de bioconjugados de interesse para a saúde humana
• Beneficiário: Giovana Cappio Barazzone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/50413-8 - Administração pulmonar de uma vacina de mucosa para pneumonia
• Beneficiário: Viviane Maimoni Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Regular