E-cig might cause cell damage of oral mucosa

Autor(es)

Tavares Schwarzmeier, Ligia Angelo ^[1] ; da Cruz, Beatriz Serralheiro ^[1] ; Panisello Ferreira, Camila Cristina ^[1] ; do Carmo Carvalho, Bruna Fernandes ^[1] ; Oliveira Alves, Monica Ghislaine ^{[2, 3]} ; Lima Carta, Celina Faig ^[1] ; Scholz, Jaqueline Ribeiro ^[4] ; Almeida, Janete Dias ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Inst Sci & Technol, Dept Biosci & Diag, Sao Jose Dos Campos - Brazil

^[2] Univ Mogi das Cruzes, Sch Dent, Mogi Das Cruzes, SP - Brazil

^[3] Univ Anhembi Morumbi, Sch Med, Sao Jose Dos Campos, SP - Brazil

^[4] Univ Sao Paulo FMUSP, Fac Med, Heart Inst Incor, Outpatient Smoking Treatment Program, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 131, n. 4, p. 435-443, APR 2021.
• Citações Web of Science: 0

Resumo

Objective. The aim of this study was to investigate cytogenetic and cytotoxic damage through the evaluation of micronuclei (MN) and metanuclear anomalies in the oral mucosa of electronic cigarette (e-cig) users. Study Design. The patients were recruited into 4 groups: e-cig users, smokers, former smokers, and nonsmokers (control). The samples were collected by means of exfoliative cytology of the lateral region of the tongue and the floor of the mouth. The smears obtained were fixed and stained by the Feulgen method for investigation of MN and metanuclear anomalies. Results. A significant difference was observed for MN frequency only between the smoker and control groups. As for metanuclear anomalies, significant differences were observed: karyolysis between: smokers and control, e-cig and control, as well as former smokers; karyorrhexis: between smoker and control; binucleation: between e-cig and former smoker, as well as control; broken eggs: between e-cig and all other groups; nuclear bud: between e-cig and former smokers, as well as control. Conclusions. E-cig and alcohol users presented genotoxicity and cytotoxicity in the oral mucosa cells. The use of e-cigs and alcohol by former smokers can cause more damage to the cells of the oral mucosa compared to those who have not used e-cigs. (AU)

• Processo FAPESP: 17/23179-7 - Estudo da expressão de genes de MLH3 e ATM em carcinoma de células escamosas bucal
• Beneficiário: Camila Cristina Panisello Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/24047-4 - Associação da ploidia do DNA e a frequência de micronúcleos na mucosa bucal em pacientes fumantes crônicos
• Beneficiário: Beatriz Serralheiro da Cruz
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/08633-0 - Tabagismo: dos aspectos moleculares a abordagem do fumante
• Beneficiário: Janete Dias Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular