Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage

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Brindo da Cruz, Isabele Camargo ^[1] ; Velosa, Ana Paula Pereira ^[1] ; Carrasco, Solange ^[1] ; dos Santos Filho, Antonio ^[1] ; Tomaz de Miranda, Jurandir ^[1] ; Pompeu, Eduardo ^[2] ; Fernandes, Tiago Lazzaretti ^{[3, 4]} ; Bueno, Daniela Franco ^[3] ; Fanelli, Camila ^[5] ; Goldenstein-Schainberg, Claudia ^[1] ; Fabro, Alexandre Todorovic ^{[6, 7]} ; Fuller, Ricardo ^[1] ; Silva, Pedro Leme ^{[8, 9]} ; Capelozzi, Vera Luiza ^[6] ; Teodoro, Walcy Rosolia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Fac Med, Rheumatol Div, FMUSP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Bioterism Ctr, FMUSP, Sao Paulo - Brazil

^[3] Hosp Sirio Libanes, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Hosp Clin, Fac Med, Sport Med Div, Inst Orthopaed & Traumatol, FMUSP, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Renal Div, Lab Cellular Genet & Mol Nephrol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Pathol, FMUSP, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, Resp Med Lab, Sao Paulo - Brazil

^[8] Natl Inst Sci & Technol Regenerat Med, Rio De Janeiro - Brazil

^[9] Univ Fed Rio de Janeiro, Carlos Chagas Filho Biophys Inst, Ctr Ciencias Saude, Lab Pulm Invest, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 9, MAR 22 2021.
• Citações Web of Science: 0

Resumo

Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H\&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment. (AU)

• Processo FAPESP: 18/20403-6 - Marcadores biomoleculares de proliferação e remodelamento em doenças respiratórias agudas e crônicas: promissores alvos terapêuticos
• Beneficiário: Vera Luiza Capelozzi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/11419-5 - Efeito terapêutico da administração de células-tronco mesenquimais estimuladas com colágeno V na cartilagem articular de coelhos com osteoartrite
• Beneficiário: Isabele Camargo Brindo da Cruz
• Modalidade de apoio: Bolsas no Brasil - Mestrado