Low-Dose Coconut Oil Supplementation Induces Hypothalamic Inflammation, Behavioral Dysfunction, and Metabolic Damage in Healthy Mice

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Veras, Alana Carolina Costa ^[1] ; dos Santos, Tamires ^[1] ; Martins, Isis de Cassia Alves ^[1] ; de Souza, Camilla Mendes ^[1] ; Amaral, Camila Libardi ^[1] ; Franco, Beatriz da Silva ^[2] ; Holanda, Alessandro Spencer de Souza ^[2] ; Esteves, Andrea Maculano ^[2] ; Milanski, Marciane ^{[3, 1]} ; Torsoni, Adriana Souza ^{[3, 1]} ; Ignacio-Souza, Leticia Martins ^{[3, 1]} ; Torsoni, Marcio Alberto ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Metab Disorders, Sch Appl Sci, Limeira - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci, Lab Sleep & Exercise, Limeira - Brazil

^[3] Univ Estadual Campinas, Obes & Comorbid Res Ctr, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR NUTRITION & FOOD RESEARCH; v. 65, n. 10 APR 2021.
• Citações Web of Science: 0

Resumo

Scope Coconut oil (CO) diets remain controversial due to the possible association with metabolic disorder and obesity. This study investigates the metabolic effects of a low amount of CO supplementation. Methods and Results Swiss male mice are assigned to be supplemented orally during 8 weeks with 300 mu L of water for the control group (CV), 100 or 300 mu L of CO (CO100 and CO300) and 100 or 300 mu L of soybean oil (SO; SO100 and SO300). CO led to anxious behavior, increase in body weight gain, and adiposity. In the hypothalamus, CO and SO increase cytokines expression and pJNK, pNFKB, and TLR4 levels. Nevertheless, the adipose tissue presented increases macrophage infiltration, TNF-alpha and IL-6 after CO and SO consumption. IL-1B and CCL2 expression, pJNK and pNFKB levels increase only in CO300. In the hepatic tissue, CO increases TNF-alpha and chemokines expression. Neuronal cell line (mHypoA-2/29) exposed to serum from CO and SO mice shows increased NFKB migration to the nucleus, TNF-alpha, and NFKBia expression, but are prevented by inhibitor of TLR4 (TAK-242). Conclusions These results show that a low-dose CO changes the behavioral pattern, induces inflammatory pathway activation, TLR4 expression in healthy mice, and stimulates the pro-inflammatory response through a TLR4-mediated mechanism. (AU)

• Processo FAPESP: 16/23484-1 - Participação de receptores colinérgicos do tipo nicotínico alfa7 (alfa7nAChR) na prevenção do desenvolvimento de resistência à insulina
• Beneficiário: Marcio Alberto Torsoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/01863-6 - Papel do receptor nicotínico colinérgico alfa 7 (alfa7nAChR) no controle hipotalâmico da homeostase energética e associação com a sinalização da leptina em camundongos
• Beneficiário: Camilla Mendes de Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado