Effects of sodium valproate on the chromatin of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae) under in vitro culture conditions

Autor(es)

Bassani, Alessandra ^[1] ; Rocha, Marina Amorim ^[1] ; Rodrigues, Vera Lucia C. C. ^[2] ; Santos, Douglas S. ^[1] ; Nascimento, Juliana Damieli ^[3] ; da Rosa, Joao Aristeu ^[3] ; Mello, Maria Luiza S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas Unicamp, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil

^[2] Superintendence Control Endem Dis SUCEN, BR-13845206 Mogi Guacu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA HISTOCHEMICA; v. 123, n. 3 APR 2021.
• Citações Web of Science: 0

Resumo

Sodium valproate (VPA) is a classic anticonvulsive, a histone deacetylase inhibitor, and a chromatin remodeling inducer. When injected into specimens of Triatoma infestans, a vector of Chagas disease, VPA affects the chromatin supraorganization of chromocenter heterochromatin in only a few cells of the Malpighian tubules. To test whether this result was explained by the inaccessibility of all of the organ's cells to the drug, we investigated the nuclear phenotypes and global acetylation of lysine 9 in histone H3 (H3K9ac) in Malpighian tubules cultivated in vitro for 1-24 h in the presence of 0.05 mM-1 mM VPA. The present results revealed that the chromatin decondensation event in the chromocenter body, which was detected only under low VPA concentrations up to a 4-h treatment, was not frequent during organ culture, similar to the results for injected insects. Cultivation of T. infestans Malpighian tubules in vitro for 24 h revealed inadequate for cell preservation even in the absence of the drug. Immunofluorescence signals for H3K9ac following VPA treatment showed a slightly increased intensity in the euchromatin, but were never detected in the chromocenter bodies, except with great intensity at their periphery, where the 18S rDNA is located. In conclusion, when VPA affects the chromocenter heterochromatin in this animal cell model, it occurs through a pathway that excludes a classic global H3K9ac mark. Investigation of nonhistone proteins associated with histone methylation marks is still required to further explain the differential response of T. infestans chromatin to VPA. (AU)

• Processo FAPESP: 18/07888-0 - Ação do Ácido Valproico (VPA) sobre a cromatina de células de túbulos de Malpighi de Triatoma infestans cultivados in vitro
• Beneficiário: Alessandra Bassani
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/10356-2 - Atuação do ácido valproico ao nível estrutural e funcional da cromatina
• Beneficiário: Maria Luiza Silveira Mello
• Modalidade de apoio: Auxílio à Pesquisa - Temático