Global transcriptome analysis implicates cholesterol availability in the regulation of canine cyclic luteal function

Autor(es)

Mattoso Miskulin Cardoso, Ana Paula ^[1] ; Pereira, Miguel Tavares ^[2] ; Silva, Renata Dos Santos ^[1] ; Medeiros de Carvalho Sousa, Liza Margareth ^[3] ; Giometti, Ines Cristina ^[4] ; Kowalewski, Mariusz Pawel ^[2] ; Papa, Paula de Carvalho ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[2] Univ Zurich, Vetsuisse Fac, Inst Vet Anat, Zurich - Switzerland

^[3] Univ Paulista, Inst Hlth Sci, Sao Paulo - Brazil

^[4] Univ Western Sao Paulo, Fac Vet Med, Presidente Prudente - Brazil

• Tipo de documento: Artigo Científico
• Fonte: General and Comparative Endocrinology; v. 307, JUN 1 2021.
• Citações Web of Science: 0

Resumo

Considering the key role of the corpus luteum in the regulation of the canine diestrus, the present study aimed to investigate changes in the luteal transcriptome of pseudopregnant dogs (n = 18) from days (D) 10, 20, 30, 40, 50 and 60 post-ovulation. After RNAsequencing was performed, data was analyzed by resorting to several informatic tools. A total of 3300 genes were differently expressed among all samples (FDR < 0.01). By comparing different time points, enriched biological processes as response to estradiol and lipids (D20 vs D10) and intra-cellular cholesterol transport (D40 vs D60) were observed. Moreover, LXR/RXR (liver X receptor- retinoid X receptor) signaling appeared as an overrepresented pathway in all comparisons. Thus, the expression of 19 genes involved in intracellular cholesterol availability was further evaluated; most were affected by time (P < 0.05). Adding to the deep transcriptomic analysis, presented data implies the importance of cholesterol regulation in luteal physiology of pseudopregnant dogs. (AU)

• Processo FAPESP: 14/00739-9 - Sinalização da insulina no corpo lúteo bovino e canino sob influência de concentrações altas e baixas de 17²-estradiol
• Beneficiário: Paula de Carvalho Papa Keohane
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/22173-9 - Correlação dos parâmetros endócrinos e comportamentais em cães abandonados em programa de re-socialização
• Beneficiário: Paula de Carvalho Papa Keohane
• Modalidade de apoio: Auxílio à Pesquisa - Regular