Ecotoxicological evaluation of water from the Sorocaba River using an integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802)

Autor(es)

Fernandes, Isabela Ferreira ^{[1, 2]} ; Moraes Utsunomiya, Heidi Samantha ^[2] ; de Lacerda Valverde, Bruno Serra ^[3] ; Cassiel Ferraz, Joao Victor ^[2] ; Fujiwara, Gabriel Hiroshi ^[2] ; Gutierres, Davi Marques ^[2] ; de Oliveira, Classius ^[4] ; Belussi, Lilian Franco ^[5] ; Fernandes, Marisa Narciso ^[6] ; Carvalho, Cleoni dos Santos ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Programa Posgrad Biotecnol & Monitoramento Ambien, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Lab Bioquim & Microbiol LaBioM, Campus Sorocaba, Rodovia Joao Leme Dos Santos, BR-264 Sorocaba, SP - Brazil

^[3] Univ Estadual Paulista, Programa Posgrad Biol Anim UNESP, Campus Sao Jose Do Rio Preto, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Fed Mato Grosso Do Sul, Inst Biociencias, Lab Patol Expt LAPex, Mato Grosso, MG - Brazil

^[6] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Caixa Postal 676, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 275, JUL 2021.
• Citações Web of Science: 0

Resumo

Lithobates catesbeianus tadpoles were exposed for 96 h to water from two sites of the Sorocaba River (summer and winter), Ibiuna (PI) and Itupararanga reservoir (PIR) that contained metals. In the liver, in PI, the glutathione peroxidase (GPx) decreased, and the glutathione S-transferase (GST) and carbonyl proteins (PCO) increased. In PIR, the glutathione reduced (GSH) increased, while there was a decrease in catalase (CAT), GPx, GST, PCO, and superoxide dismutase (SOD). In winter, GPx and GST increased in both points. Regarding the kidneys, lipoperoxidation (LPO) levels and GST decreased, while GSH increased in the summer. In the winter, LPO increased in PI. In the muscle, in the summer, there was an increase in GSH and GST and change in PCO. In the winter, the levels of PCO increased and CAT decreased in PIR. The area and volume of the hepatocyte and nucleus area increased in the summer and decreased in the winter. Hepatic melanin decreased in the summer after exposure to PIR water. There were the systemic effects of Sorocaba River water exposure at different times of the year with alterations in biomarkers at different levels, in which kidney shows highest Integrated Response of Biomarkers (IBR) value followed by liver and muscle. Biochemical biomarkers were more sensitive than morphological ones. The more sensitive biochemical markers were MT, PCO, GST and LPO. These effects confirm the hypothesis of metabolic alteration in bullfrog tadpoles by the Sorocaba River water. (C) 2021 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 17/23781-9 - Avaliação ecotoxicológica da água do rio Sorocaba utilizando uma análise integrada de biomarcadores bioquímicos e morfológicos em girinos de rã-touro, Lithobates catesbeianus (Shaw, 1802).
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular