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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Low-fouling properties in serum of carboxylic-oligo(ethylene glycol)-based interfaces

Texto completo
Autor(es):
Santos, Adriano [1] ; Garcia Nicholson, Melany Isabel [1] ; Feliciano, Gustavo Troiano [1] ; Bueno, Paulo Roberto [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Chem, Sao Paulo State Univ, BR-14800060 Araraquara, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 618, JUN 5 2021.
Citações Web of Science: 0
Resumo

In the present work we evaluated the low-fouling feature of a carboxylic-oligo(ethylene glycol) self-assembled monolayer (carboxylic-OEG-SAM) interface using a quartz crystal microbalance with dissipation monitoring (QCM-D). QCM-D measurements allowed us to estimate the amount of protein loading in two different serum dilutions at two different interfaces: bare gold and carboxylic-OEG. The observed amount of protein adsorbed onto bare gold is about twice higher that of carboxylic OEG interface, confirming the low-fouling characteristics of OEG-modified surfaces. Additionally, QCM-D results demonstrated the existence of two protein adsorption regimes, a faster and a slower, with distinct dissipation energies which was modelled by a two-step kinetic model. The faster regime was attributed to the adsorption of proteins into free sites of the carboxylic-OEG-SAM in a rigid binding process, followed by a slower and more viscoelastic adsorption process ascribed to structural conformational changes; this slower step conforms with the filling of remaining free sites associated with the steric hindrance in which protein-protein interactions defines the slower rate constant for the adsorption. (AU)

Processo FAPESP: 17/24839-0 - Eletrônica e eletroquímica em escala nanométrica: fundamentos e aplicações
Beneficiário:Paulo Roberto Bueno
Modalidade de apoio: Auxílio à Pesquisa - Temático