Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles

Autor(es)

Seriani, Robson ^{[1, 2]} ; de Paula, Carla Peres ^[3] ; da Cunha, Anderson Ferreira ^[3] ; de Oliveira, Marcos Antonio ^[4] ; Krempel, Paloma Gava ^[5] ; Frias, Daniela Perroni ^[1] ; Negri, Elnara Marcia ^[1] ; Mauad, Thais ^[1] ; Macchione, Mariangela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Expt Air Pollut LIM05, FMUSP Ave Dr Arnaldo 455, 1 Floor, Room 1150, BR-01246903 Sao Paulo, SP - Brazil

^[2] PROSCED Consulting Training & Dev, Rua Alto Belo, 742, Room 01, BR-03478040 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Carlos, Genet & Evolut Dept, UFSCar Ave Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Sao Paulo State Univ, Struct & Funct Mol Biol, Biosci Inst, Coastal Campus, BR-11330900 Sao Vicente, SP - Brazil

^[5] Univ Sao Paulo, Med Sch Sao Paulo, Lab Invest Ophthalmol LIM 33, FMUSP Dr Arnaldo 455, 5 Floorr, BR-01246903 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental and Molecular Pathology; v. 120, JUN 2021.
• Citações Web of Science: 0

Resumo

Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 mu g/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 +/- 0.34; {*}p = 0.0220), PRDX5 (0.43 +/- 0.34; {*}p = 0.0220), and PRDX6 (0.33 +/- 0.25; {*}p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions. (AU)

• Processo FAPESP: 11/50334-7 - Avaliação da toxicidade do material particulado urbano em cultura de células do epitélio brônquico humano: estudo da expressão gênica de enzimas antioxidante e sinalização celular
• Beneficiário: Robson Seriani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto