Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice

Autor(es)

Silva, Heloina Nathallie Mariano da ^[1] ; Covatti, Caroline ^[1] ; Rocha, Guilherme Luiz da ^[1] ; Mizobuti, Daniela Sayuri ^[1] ; Mancio, Rafael Dias ^[1] ; Hermes, Tulio de Almeida ^[1] ; Kido, Larissa Akemi ^[1] ; Cagnon, Valeria Helena Alves ^[1] ; Pereira, Elaine Cristina Leite ^{[2, 1]} ; Minatel, Elaine ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Dept Struct & Funct Biol, Campinas - Brazil

^[2] Univ Brasilia UNB, Fac Ceilandia, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PHYSIOLOGY; v. 12, APR 26 2021.
• Citações Web of Science: 0

Resumo

The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7-11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-kappa B and increased the PGC1-alpha and PPAR delta levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD. (AU)

• Processo FAPESP: 17/01638-0 - Tempol: potencial terapia antioxidante para fibras musculares distróficas
• Beneficiário: Elaine Minatel
• Modalidade de apoio: Auxílio à Pesquisa - Regular