Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients

Autor(es)

Peiro, Carlos Henrique F. ^[1] ; Perez, Matheus M. ^[1] ; de Aquino, Glauco S. A. ^[1] ; Encinas, Jessica F. A. ^[1] ; Sousa, Luiz Vinicius de A. ^[2] ; da Veiga, Glaucia Luciano ^[1] ; del Giglio, Auro ^[3] ; Fonseca, Fernando L. A. ^{[1, 4]} ; da Costa Aguiar Alves, Beatriz ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Univ Saude ABC, Fac Med ABC, Lab Anal Clin, Av Lauro Gomes 2000, BR-09060870 Santo Andre, SP - Brazil

^[2] FMABC, Lab Epidemiol & Anal Dados, Santo Andre, SP - Brazil

^[3] Ctr Univ Saude ABC, Fac Med ABC, Dept Oncol & Hematol, Santo Andre, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Farmaceut, Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 APR 22 2021.
• Citações Web of Science: 0

Resumo

In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer patients in order to verify their predictive potential in liquid biopsies. Gene expressions were analyzed by qPCR in tumor and blood samples from 125 patients and 25 healthy women. Differential expression was determined by the 2(-Delta Cq) method. Expression of HIF-1 alpha and GLUT1 in the blood of breast cancer patients is significantly higher (90-91 and 160-161 fold increased expression, respectively; p < 0.0001) than that found in healthy women. Their diagnostic power was confirmed by ROC curve. CAIX is also more expressed in breast cancer women blood, but its expression was detected only in a few samples. But none of these genes could be considered predictive markers. Therefore, evaluation of the expression of HIF-1 alpha and GLUT1 in blood may be a useful laboratory tool to complement the diagnosis of breast cancer, in addition to being useful for follow-up of patients and of women with a family history of breast cancer. (AU)

• Processo FAPESP: 17/03558-3 - Determinação do potencial prognóstico de genes induzidos pela hipóxia (HIF, GLUT1 e CAIX) em pacientes com câncer de mama.
• Beneficiário: Beatriz da Costa Aguiar Alves Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular