Antigenotoxic potential of the fermentation broth produced by Paenibacillus polymyxa RNC-D in vitro

Autor(es)

Bianchi, Jaqueline ^[1] ; Cavicchioli, Rafael ^[1] ; Kubota, Lauro T. ^{[2, 3]} ; Carrilho, Emanuel ^{[3, 4]} ; de Sousa, Cristina P. ^[1] ; Freitas Anibal, Fernanda de ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Ctr Ciencias Biol & Saude, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[4] Inst Nacl Ciencia & Tecnol Bioanal INCTBio, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 16, n. 7, p. 471-485, MAY 2021.
• Citações Web of Science: 0

Resumo

Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 mu g/ml concentrations induced DNA lesions, whereas the 4 mu g/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-alpha was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-alpha release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound. (AU)

• Processo FAPESP: 16/13423-5 - Compostos produzidos por microrganismos endofíticos isolados de plantas do cerrado: bioatividade, associação com a-Ag2WO4 e aplicações biotecnológicas
• Beneficiário: Cristina Paiva de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático