| Full text | |
| Author(s): |
Bianchi, Jaqueline
[1]
;
Cavicchioli, Rafael
[1]
;
Kubota, Lauro T.
[2, 3]
;
Carrilho, Emanuel
[3, 4]
;
de Sousa, Cristina P.
[1]
;
Freitas Anibal, Fernanda de
[3, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Carlos UFSCar, Ctr Ciencias Biol & Saude, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Quim, BR-13083970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[4] Inst Nacl Ciencia & Tecnol Bioanal INCTBio, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FUTURE MICROBIOLOGY; v. 16, n. 7, p. 471-485, MAY 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 mu g/ml concentrations induced DNA lesions, whereas the 4 mu g/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-alpha was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-alpha release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound. (AU) | |
| FAPESP's process: | 16/13423-5 - Compounds produced by endophyte microorganisms isolated from Brazilian tropical savannah: bioactivity, association with a-Ag2WO4 and biotechnological applications |
| Grantee: | Cristina Paiva de Sousa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |