Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae

Autor(es)

Martins, Leandro Freitas ^[1] ; Hilbig, Cleonice Cristina ^[1] ; Yasui, George Shigueki ^[2] ; Monzani, Paulo Sergio ^[2] ; Senhorini, Jose Augusto ^[3] ; Okada Nakaghi, Laura Satiko ^[3] ; do Nascimento, Nivaldo Ferreira ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Fed Univ Grande Dourados UFGD, BR-79825070 Dourados, MS - Brazil

^[2] Chico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP - Brazil

^[3] Sao Paulo State Univ, Aquacuiture Ctr, Via Acesso Prof Paulo Donato Castaane S-N, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZYGOTE; v. 29, n. 1, p. 82-86, FEB 2021.
• Citações Web of Science: 0

Resumo

The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40 degrees C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22 degrees C, 26 degrees C and 28 degrees C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species. (AU)

• Processo FAPESP: 16/12383-0 - Técnicas de manipulação cromossômica no lambari (Astyanax altiparanae)
• Beneficiário: Nivaldo Ferreira do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/11664-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 10/17429-1 - Quimerismo em espécies nativas de peixes como ferramenta para a propagação mediada na aquicultura
• Beneficiário: George Shigueki Yasui
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores