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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting

Texto completo
Autor(es):
Ruks, Tatjana [1, 2] ; Loza, Kateryna [1, 2] ; Heggen, Marc [3] ; Prymak, Oleg [1, 2] ; Sehnem, Andre Luiz [4] ; Oliveira, Cristiano L. P. [4] ; Bayer, Peter [5] ; Beuck, Christine [5] ; Epple, Matthias [1, 2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Duisburg Essen, Inorgan Chem, D-45117 Essen - Germany
[2] Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45117 Essen - Germany
[3] Forschungszentrum Julich, Ernst Ruska Ctr Microscopy & Spect Electrons, D-52425 Julich - Germany
[4] Univ Sao Paulo, Inst Phys, BR-05508090 Sao Paulo - Brazil
[5] Univ Duisburg Essen, Ctr Med Biotechnol ZMB, Dept Struct & Med Biochem, D-45117 Essen - Germany
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: ACS APPLIED BIO MATERIALS; v. 4, n. 1, p. 945-965, JAN 18 2021.
Citações Web of Science: 0
Resumo

Ultrasmall gold nanoparticles with a metallic core diameter of 2 nm were surface-conjugated with peptides that selectively target epitopes on the surface of the WW domain of the model protein hPin1 (hPin1-WW). The binding to the gold surface was accomplished via the thiol group of a terminal cysteine. The particles were analyzed by NMR spectroscopy, high-resolution transmission electron microscopy, and differential centrifugal sedimentation. The surface loading was determined by conjugating a FAM-labeled peptide, followed by UV-vis spectroscopy, and by quantitative H-1 NMR spectroscopy, showing about 150 peptide molecules conjugated to each nanoparticle. The interaction between the peptide-decorated nanoparticles with hPin1-WW was probed by H-1-N-15-HSQC NMR titration, fluorescence polarization spectroscopy (FP), and isothermal titration calorimetry (ITC). The particles showed a similar binding (K-D = 10-20 mu M) compared to the dissolved peptides (K-D = 10-30 mu M). Small-angle X-ray scattering (SAXS) showed that the particles were well dispersed and did not agglomerate after the addition of hPin1-WW (no cross-linking by the protein). Each nanoparticle was able to bind about 20 hPin1-WW protein molecules. An unspecific interaction with hPin1 was excluded by the attachment of a nonbinding peptide to the nanoparticle surface. The uptake by cells was studied by confocal laser scanning microscopy. The peptide-fiinctionalized nanoparticles penetrated the cell membrane and were located in the cytosol. In contrast, the dissolved peptide did not cross the cell membrane. Peptide-functionalized nanoparticles are promising agents to target proteins inside cells. (AU)

Processo FAPESP: 19/10433-8 - Simulação e Modelagem Estrutural de Cristais Líquidos orientados utilizando Métodos de Espalhamento a Baixos Ângulos
Beneficiário:André Luiz Sehnem
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 19/06750-8 - Investigando a interação não-covalente de nanopartículas de ouro ultra-pequenas com proteínas selecionadas
Beneficiário:Cristiano Luis Pinto de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/16092-5 - Investigando sistemas coloidais por métodos de espalhamento
Beneficiário:Cristiano Luis Pinto de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/24531-3 - Propriedades estruturais e biofísicas de lipoproteínas nativa e modificada
Beneficiário:Antonio Martins Figueiredo Neto
Modalidade de apoio: Auxílio à Pesquisa - Temático