Increased expression of interleukin-6 gene in gastritis and gastric cancer

Autor(es)

Santos, M. P. ^[1] ; Pereira, J. N. ^[1] ; Delabio, R. W. ^[1] ; Smith, M. A. C. ^[2] ; Payao, S. L. M. ^[1] ; Carneiro, L. C. ^[3] ; Barbosa, M. S. ^[3] ; Rasmussen, L. T.

Afiliação do(s) autor(es)

^[1] Fac Med Marilia, Lab Genet, Marilia, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, SP - Brazil

^[3] Univ Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 54, n. 7 2021.
• Citações Web of Science: 0

Resumo

Helicobacter pylori (H. pylori) induces an intense inflammatory response, mediated by proinflammatory cytokines, including interleukin (IL)-6 and its membrane receptor (IL-6R), which activates important signaling pathways in the development of gastric disease and cancer. We investigated the gene and protein expression of IL-6 and IL-6R and the influence of polymorphisms rs1800795, rs1800796, and rs1800797 on its gene expression together with H. pylori infection. Furthermore, an in-silico analysis was performed to support our results. Gastric biopsies were obtained from patients with gastric symptoms and patients with gastric cancer (GC) and were divided into groups (Control, Gastritis, and Cancer). H. pylori was detected by PCR. Real-time-qPCR was employed to determine gene expression, and western blot assay was used to analyze protein expression levels. PCR-RFLP was used to characterize IL-6 polymorphisms. Bioinformatics analyses were performed using the Gene Expression Omnibus (GEO) database and GEO2R to screen out differentially expressed genes (DEGs). H. pylori was detected in 43.3% of the samples. Statistically significant differences were found for IL-6 (P=0.0001) and IL-6R (P=0.0005) genes among the three groups, regardless of the presence of H. pylori. Among patients with H. pylori infection, the IL-6 and IL-6R gene and protein expressions were significantly increased, highlighting IL-6 gene overexpression in patients with GC. No statistically significant differences were found for the rs1800795, rs1800796, and rs1800797 polymorphisms compared to IL-6 gene expression. The results indicated that the IL-6 polymorphisms do not influence its expression, but IL-6 and IL-6R expression seems to be altered by the presence of H. pylori. (AU)

• Processo FAPESP: 16/25562-0 - Abordagem molecular e funcional no câncer gástrico da população brasileira
• Beneficiário: Marilia de Arruda Cardoso Smith
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/02008-2 - Análise da expressão dos micros RNAs miR-let7a, miR-125a, miR-155 e dos genes da interleucina 6 e seu receptor-alpha em pacientes com doenças gástricas e cultura celular: uma possível associação com o H. pylori
• Beneficiário: Monica Pezenatto dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/08481-1 - Análise da Interação Gênica e Proteica entre Componentes das Vias Inflamatória, da Apoptose e MicroRNAs em Doença Dispéptica e Câncer Gástrico
• Beneficiário: Lucas Trevizani Rasmussen
• Modalidade de apoio: Auxílio à Pesquisa - Regular