Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Autor(es)

Godoi, Filipe G. Andrade ^{[1, 2]} ; Forner-Piquer, Isabel ^[1] ; Randazzo, Basilio ^[1] ; Habibi, Hamid R. ^[3] ; Lo Nostro, Fabiana L. ^{[4, 5]} ; Moreira, Renata Guimaraes ^[2] ; Carnevali, Oliana ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Politecn Marche, Dipartimento Sci Vita & Ambiente, Ancona - Italy

^[2] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, Rua Matao, Sao Paulo - Brazil

^[3] Univ Calgary, Dept Biol Sci, Calgary, AB - Canada

^[4] Univ Buenos Aires, UBA, CONICET, Lab Ecotoxicol Acuat, IBBEA, Buenos Aires, DF - Argentina

^[5] Univ Buenos Aires, FCEyN, DBBE, Buenos Aires, DF - Argentina

^[6] Consorzio Interuniv Biosistemi & Biostrutture, Ist Nazl Biostrutture Biosistemi, Rome - Italy

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN ENDOCRINOLOGY; v. 12, JUN 14 2021.
• Citações Web of Science: 0

Resumo

Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations {[}0.42 mu g L-1 (10(-9) M), 4.2 mu g L-1 (10(-8) M), and 42 mu g L-1 (10(-7) M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 mu g L-1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 mu g L-1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 mu g L-1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner. (AU)

• Processo FAPESP: 17/07139-5 - Ajustes na fisiologia reprodutiva de machos Astyanax altiparanae (Teleostei: Characidae) expostos a contaminantes emergentes
• Beneficiário: Filipe Guilherme Andrade de Godoi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 18/10495-0 - Di-isononyl phthalato como um contaminante emergente na água doce: quais são os riscos para os processos apoptóticos e autofágicos nas gônadas do zebrafish
• Beneficiário: Filipe Guilherme Andrade de Godoi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 17/11530-1 - Ajustes fisiológicos de teleósteos tropicais em reservatórios impactados
• Beneficiário: Renata Guimarães Moreira Whitton
• Modalidade de apoio: Auxílio à Pesquisa - Regular