Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Author(s)

Godoi, Filipe G. Andrade ^{[1, 2]} ; Forner-Piquer, Isabel ^[1] ; Randazzo, Basilio ^[1] ; Habibi, Hamid R. ^[3] ; Lo Nostro, Fabiana L. ^{[4, 5]} ; Moreira, Renata Guimaraes ^[2] ; Carnevali, Oliana ^{[1, 6]}

Affiliation

^[1] Univ Politecn Marche, Dipartimento Sci Vita & Ambiente, Ancona - Italy

^[2] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, Rua Matao, Sao Paulo - Brazil

^[3] Univ Calgary, Dept Biol Sci, Calgary, AB - Canada

^[4] Univ Buenos Aires, UBA, CONICET, Lab Ecotoxicol Acuat, IBBEA, Buenos Aires, DF - Argentina

^[5] Univ Buenos Aires, FCEyN, DBBE, Buenos Aires, DF - Argentina

^[6] Consorzio Interuniv Biosistemi & Biostrutture, Ist Nazl Biostrutture Biosistemi, Rome - Italy

• Document type: Journal article
• Source: FRONTIERS IN ENDOCRINOLOGY; v. 12, JUN 14 2021.
• Web of Science Citations: 0

Abstract

Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations {[}0.42 mu g L-1 (10(-9) M), 4.2 mu g L-1 (10(-8) M), and 42 mu g L-1 (10(-7) M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 mu g L-1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 mu g L-1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 mu g L-1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner. (AU)

• FAPESP's process: 17/07139-5 - Adjustments on males reproductive physiology of Astyanax altiparanae (Teleostei:Characidae) exposed to emerging compounds
• Grantee: Filipe Guilherme Andrade de Godoi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
• Grantee: Carlos Arturo Navas Iannini
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants
• FAPESP's process: 18/10495-0 - Di-isononyl phthalate an emerging contaminant in freshwater: what are the risks for apoptotic and autophagyc processes in zebrafish gonads
• Grantee: Filipe Guilherme Andrade de Godoi
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/11530-1 - Physiological adjustments of tropical teleosts in anthropogenic reservoirs
• Grantee: Renata Guimarães Moreira Whitton
• Support Opportunities: Regular Research Grants