Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil

Autor(es)

Cardozo, Marita Vedovelli ^[1] ; Liakopoulos, Apostolos ^{[2, 3]} ; Brouwer, Michael ^[2] ; Kant, Arie ^[2] ; Luduverio Pizauro, Lucas Jose ^[4] ; Borzi, Mariana Monezi ^[1] ; Mevius, Dik ^[2] ; de Avila, Fernando Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Fac Agr & Vet Sci, Dept Vet Pathol, Jaboticabal - Brazil

^[2] Wageningen Univ & Res, Dept Bacteriol & Epidemiol, Wageningen Biovet Res, Lelystad - Netherlands

^[3] Leiden Univ, Inst Biol Leiden, Dept Microbial Biotechnol & Hlth, Leiden - Netherlands

^[4] Sao Paulo State Univ Unesp, Fac Agr & Vet Sci, Dept Tecnol, Jaboticabal - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 12, JUN 22 2021.
• Citações Web of Science: 0

Resumo

This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the bla(CTX-M-1) or bla(CTX-M-2) gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The bla(CTX-M) genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of bla(CTX-M-15) among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America. (AU)

• Processo FAPESP: 21/03188-7 - Ocorrência e caracterização molecular de Enterobactérias produtoras de beta lactamases de espectro estendido em amostras de frango, carne de frango e humanos no estado de São Paulo, Brasil
• Beneficiário: Fernando Antonio de Avila
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 15/10140-0 - Genes de resistência e tipagem plasmidial de Enterobacteriaceae produtoras de betalactamase de espectro estendido de isolados de aves e de humanos do Brasil
• Beneficiário: Marita Vedovelli Cardozo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/18280-0 - Caracterização molecular e disseminação de enterobactérias produtoras de Betalactamase de Espectro Extendido desde a produção de aves até o seu consumo
• Beneficiário: Marita Vedovelli Cardozo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado