| Full text | |
| Author(s): |
Cardozo, Marita Vedovelli
[1]
;
Liakopoulos, Apostolos
[2, 3]
;
Brouwer, Michael
[2]
;
Kant, Arie
[2]
;
Luduverio Pizauro, Lucas Jose
[4]
;
Borzi, Mariana Monezi
[1]
;
Mevius, Dik
[2]
;
de Avila, Fernando Antonio
[1]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ Unesp, Fac Agr & Vet Sci, Dept Vet Pathol, Jaboticabal - Brazil
[2] Wageningen Univ & Res, Dept Bacteriol & Epidemiol, Wageningen Biovet Res, Lelystad - Netherlands
[3] Leiden Univ, Inst Biol Leiden, Dept Microbial Biotechnol & Hlth, Leiden - Netherlands
[4] Sao Paulo State Univ Unesp, Fac Agr & Vet Sci, Dept Tecnol, Jaboticabal - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MICROBIOLOGY; v. 12, JUN 22 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the bla(CTX-M-1) or bla(CTX-M-2) gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The bla(CTX-M) genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of bla(CTX-M-15) among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America. (AU) | |
| FAPESP's process: | 15/10140-0 - Resistence genes and plasmid typing of Enterobacteriaceae producing Extended spectrum betalactamase from Brazilian poultry and human strains |
| Grantee: | Marita Vedovelli Cardozo |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 13/18280-0 - Molecular characterization and dissemination of Enterobacteriaceae producing Extended spectrum beta-lactamase from poultry production to its consumption |
| Grantee: | Marita Vedovelli Cardozo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |