Immunogenicity in Oreochromis niloticus vaccinated with sonicated antigens against streptococcosis

Autor(es)

Marcusso, Paulo Fernandes ^{[1, 2]} ; Claudiano, Gustavo da Silva ^{[2, 3, 4]} ; Yunis-Aguinaga, Jefferson ^{[4, 5, 6]} ; Marinho-Neto, Fausto de Almeida ^[2] ; Eto, Silas Fernandes ^{[2, 7]} ; Fernandes, Dayanne Carla ^{[8, 2]} ; Rosolem, Mayara Caroline ^{[2, 9]} ; Salvador, Rogerio ^[10] ; Engracia de Moraes, Julieta Rodini ^{[2, 4]} ; de Moraes, Flavio Ruas ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Jequitinhonha & Mucuri Valleys, Inst Agr Sci, Haifa - Israel

^[2] Sao Paulo State Univ UNESP, Fac Agr & Veterinarian Sci, Dept Pathol Theriogenol & One Hlth, UNESP, Jaboticabal, SP - Brazil

^[3] Fed Univ Western Para, UFOPA, Inst Biodivers & Forests, Santarem, Para - Brazil

^[4] Sao Paulo State Univ UNESP, Aquaculture Ctr, Postgrad Program Aquaculture, Jaboticabal, SP - Brazil

^[5] Sci Univ South, Lima - Peru

^[6] Sea Inst Peru, Callao - Peru

^[7] Fed Univ Roraima UFRR, Dept Postgrad Hlth Sci PROCISA, Boa Vista, Parana - Brazil

^[8] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[9] Vet Pathologist Autonomous, Sao Paulo - Brazil

^[10] State Univ Northern Parana, Bandeirantes, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FISH & SHELLFISH IMMUNOLOGY; v. 115, p. 134-141, AUG 2021.
• Citações Web of Science: 0

Resumo

Streptococcosis causes great economic losses in intensive culture of tilapia. Vaccination is the most effective and safest way to tackle infectious diseases. Thus, this study sought the more effective and safer antigenic fraction after sonication of Streptococcus agalactiae to elaborate a vaccine against streptococcosis in Nile tilapia. For this, twenty-one days after vaccination with different fractions (soluble and insoluble) of S. agalactiae, the fish were challenged with the homologous strain (LD50). Then, samples were taken at zero, 14, 28, 60 and 90 days postvaccination (DPV, n = 7). Blood and organs (cranial kidney, spleen and liver) were collected from vaccinated and unvaccinated fish. Finally, insoluble fraction vaccine presented the best effect, resulting in a 100% relative percent of survival (RPS) and without clinical manifestations. In view of the results, it was to evaluate the role of the insoluble fraction of the antigen in the protective immunity against streptococcosis. The results indicate that the spleen might be the main organ in the vaccine response in Nile tilapia due to the great morphological and immunological differences in vaccinated fish, evidenced by the greater of melanomacrophage centers (MMC) and IgM + lymphocytes in relation to the non-vaccinated fish. At 60 DPV, it was observed the peak of the protective immunity related to the maximum concentration of proteins, circulating leukocytes, antibody titers in the serum and tissue changes with greater expression of IgM + and MMC number in the spleen and kidney of Oreochromis niloticus. Vaccination with insoluble fraction of S. agalactiae was safe and provided effective protection against streptococcosis with maximum protective response at 60 DPV. (AU)

• Processo FAPESP: 13/24852-6 - AVALIAÇÃO HISTOLÓGICA E IMUNOHISTOQUÍMICA DE TILÁPIAS DO NILO (Oreochromis niloticus) VACINADAS CONTRA Streptococcus agalactiae
• Beneficiário: Paulo Fernandes Marcusso
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/07534-8 - RESPOSTA IMUNE DE TILÁPIAS DO NILO (Oreochromis niloticus) VACINADAS CONTRA Streptococcus agalactiae
• Beneficiário: Paulo Fernandes Marcusso
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/20280-2 - ASPECTOS DA FISIOPATOLOGIA DA SEPSE EM Piaractus mesopotamicus INDUZIDA POR Aeromonas hydrophila
• Beneficiário: Gustavo da Silva Claudiano
• Modalidade de apoio: Bolsas no Brasil - Doutorado