Pangenome analyses of LuxS-coding genes and enzymatic repertoires in cocoa-related lactic acid bacteria

Autor(es)

Goncalves de Almeida, Otavio Guilherme ^[1] ; Vitulo, Nicola ^[2] ; De Martinis, Elaine Cristina Pereira ^[1] ; Felis, Giovanna E. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Verona, Dept Biotechnol, Verona - Italy

• Tipo de documento: Artigo Científico
• Fonte: Genomics; v. 113, n. 4, p. 1659-1670, JUL 2021.
• Citações Web of Science: 0

Resumo

Lactobacillaceae presents potential for interspecific Quorum Sensing (QS) in spontaneous cocoa fermentation, correlated with high abundance of luxS. Three Brazilian isolates from cocoa fermentation were characterized by Whole Genome Sequencing and luxS gene was surveyed in their genomes, in comparison with public databases. They were classified as Lactiplantibacillus plantarum, Limosilactobacillus fermentum and Pediococcus acidilactici. LuxS genes were conserved in core genomes of the novel isolates, but in some non-cocoa related Lactic Acid Bacteria (LAB) it was accessory and plasmid-borne. The conservation and horizontal acquisition of luxS reinforces that QS is determinant for bacterial adaptation in several environments, especially taking into account the luxS has been correlated with modulation of bacteriocin production, stress tolerance and biofilm formation. Therefore, in this paper, new clade and species-specific primers were designed for future application for screening of luxS gene in LAB to evaluate the adaptive potential to diverse food fermentations. (AU)

• Processo FAPESP: 17/13759-6 - Prospecção metagenômica de bactérias relacionadas a quorum sensing durante a fermentação espontânea do cacau
• Beneficiário: Otávio Guilherme Gonçalves de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/13564-3 - Prospecção metagenômica de bactérias relacionadas a quorum sensing durante a fermentação espontânea do cacau
• Beneficiário: Elaine Cristina Pereira de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular