Effect of enzymatic treatment of citrus by-products on bacterial growth, adhesion and cytokine production by Caco-2 cells

Autor(es)

Menezes Barbosa, Paula de Paula ^[1] ; Ruviaro, Amanda Roggia ^[2] ; Martins, Isabela Mateus ^[2] ; Macedo, Juliana Alves ^[2] ; LaPointe, Gisele ^[3] ; Macedo, Gabriela Alves ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil

^[3] Univ Guelph, Canadian Res Inst Food Safety, Dept Food Sci, Guelph, ON N1G 2W1 - Canada

• Tipo de documento: Artigo Científico
• Fonte: FOOD & FUNCTION; v. 11, n. 10, p. 8996-9009, OCT 1 2020.
• Citações Web of Science: 1

Resumo

Citrus by-products are inexpensive sources of polyphenols, important bioactive compounds with wide pharmaceutical and food applications. This study aimed to investigate the effect of enzymatic treatment of citrus by-products on the polyphenolic profile of extracts and assess the influence of extracts on the growth and adhesion of probiotics and foodborne pathogenic bacteria and on the inflammatory response of epithelial cells. Enzyme-assisted extraction altered the polyphenolic profile (as assessed by HPLC-DAD), increasing the content of aglycone flavanones (naringenin and hesperetin). Enzymatic extracts and aglycone flavanones exhibited higher antibacterial and prebiotic activities than non-enzymatic extracts and glycoside flavanones. However, a higher content of aglycones was not associated with higher anti-adhesion activity. Citrus extracts significantly (P <= 0.05) decreased the inflammatory response of Caco-2 cells toSalmonellaTyphimurium adhesion. These results support the sustainable reuse of citrus agroindustrial wastes and indicate the potential of citrus extracts in preventing infection by foodborne pathogenic bacteria and inducing proliferation of probiotics in foods and the gut environment. (AU)

• Processo FAPESP: 15/04555-2 - Desenvolvimento de processos biológicos para obtenção de extratos ricos em compostos fenólicos a partir de resíduos da indústria de cítricos e avaliação do potencial bioativo dos extratos
• Beneficiário: Gabriela Alves Macedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular