In vitro effect of curcumin-mediated antimicrobial photodynamic therapy on fibroblasts: viability and cell signaling for apoptosis

Autor(es)

Chaves Lamarque, Giuliana Campos ^[1] ; Mendez, Daniela Alejandra Cusicanqui ^[1] ; Matos, Adriana Arruda ^[2] ; Dionisio, Thiago Jose ^[2] ; Machado, Maria Aparecida Andrade Moreira ^[1] ; Magalhaes, Ana Carolina ^[2] ; Oliveira, Rodrigo Cardoso ^[2] ; Cruvinel, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Al Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 36, n. 6, p. 1169-1175, AUG 2021.
• Citações Web of Science: 0

Resumo

Although it was demonstrated that curcumin-mediated antimicrobial photodynamic therapy (aPDT) is effective for reducing the viability of microbial cells and the vitality of oral biofilms, the cytotoxicity of this therapeutic approach for host cells has not been yet elucidated. Hence, the aim of this study was to evaluate the cytotoxicity and apoptotic effects of curcumin-mediated aPDT on mouse fibroblasts. Cells were treated with 0.6 or 6 mu mol.L(-1)curcumin combined with 0.075 or 7.5 J.cm(-2)LED at 455 nm. Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet (CV) assays, while quantitative reverse transcriptase-PCR (qRT-PCR) was used to assess the expression of Bax, Bad, Bcl-2, VDAC-1, cytochrome C, and Fas-L genes for apoptosis. The differences between groups were detected by Kruskal-Wallis and post hoc Dunn's tests for MTT and CV assays and by ANOVA and post hoc Tukey test for qRT-PCR (P< 0.05). The effect of 0.6 mu mol.L(-1)curcumin plus 0.075 J.cm(-2)LED (minimum parameter) did not differ statistically from control group; however, the combination of 0.6 mu mol.L(-1)curcumin plus 7.5 J.cm(-2)LED reduced viable cells in 34%, while the combinations of 6 mu mol.L(-1)curcumin plus 0.075 and 7.5 J.cm(-2)LED reduced viable cells in 47% and 99%, respectively. aPDT increased significantly the relative expression of Bax/Bcl-2, cytochrome C, VDAC-1, and Fas-L genes, without influence on the ratio Bad/Bcl-2. Therefore, curcumin-mediated aPDT activated Bcl-2 apoptosis signaling pathways in mouse fibroblasts regarding present conditions, reducing the viability of cells with the increase of curcumin concentrations and light energies. (AU)

• Processo FAPESP: 13/20372-0 - Efeito do verniz de tetrafluoreto de titânio sobre fibroblastos (NIH3T3): ensaios de viabilidade, morfologia e sinalização celular para apoptose
• Beneficiário: Ana Carolina Magalhães
• Modalidade de apoio: Auxílio à Pesquisa - Regular