Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii

Autor(es):Mostrar menos -

Vieira de Araujo, Anna Erika ^{[1, 2]} ; Conde, Luis Vidal ^[1] ; da Silva Junior, Haroldo Cid ^[1] ; Machado, Lucas de Almeida ^[2] ; Lara, Flavio Alves ^[2] ; Chapeaurouge, Alex ^[2] ; Pauer, Heidi ^[3] ; Pires Hardoim, Cristiane Cassiolato ^[4] ; Martha Antunes, Luis Caetano ^{[2, 3]} ; D'alincourt Carvalho-Assef, Ana Paula ^[2] ; Moreno Senna, Jose Procopio ^[1]

Afiliação do(s) autor(es)

^[1] Brazilian Minist Hlth, Oswaldo Cruz Fdn, Biomanguinhos, Rio De Janeiro, RJ - Brazil

^[2] Brazilian Minist Hlth, Oswaldo Cruz Inst, Oswaldo Cruz Fdn, Rio De Janeiro, RJ - Brazil

^[3] Fundacao Oswaldo Cruz, Ctr Technol Dev Hlth, Natl Inst Sci & Technol Innovat Dis Neglected Pop, Rio De Janeiro, RJ - Brazil

^[4] Sao Paulo State Univ, Inst Biosci, Coastal Campus Sao Vicente, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbes and Infection; v. 23, n. 4-5 MAY-JUN 2021.
• Citações Web of Science: 0

Resumo

Acinetobacter baumannii is an important nosocomial pathogen. BamA is a protein that belongs to a complex responsible for organizing the proteins on the bacterial outer membrane. In this work, we aimed to evaluate murine immune responses to BamA recombinant protein (rAbBamA) from A. baumannii in an animal model of infection, and to assess cross-reactivity of this target for the development of anti -A. baumannii vaccines or diagnostics. Immunization of mice with rAbBamA elicited high antibody titers and antibody recognition of native A. baumannii BamA. Immunofluorescence also detected binding to the bacterial surface. After challenge, immunized mice demonstrated a 40% survival increase and better bacterial clearance in kidneys. Immunoblot of anti-rAbBamA against other medically relevant bacteria showed binding to proteins of approximately 35 kDa in Klebsiella pneumoniae and Escherichia coli lysates, primarily identified as OmpA and OmpC, respectively. Altogether, our data show that anti-rAbBamA antibodies provide a protective response against A. baumannii infection in mice. However, the response elicited by immunization with rAbBamA is not completely specific to A. baumannii. Although a broad-spectrum vaccine that protects against various pathogens is an appealing strategy, antibody reactivity against the human microbiota is undesired. In fact, immunization with rAbBamA produced noticeable effects on the gut microbiota. However, the changes elicited were small and non-specific, given that no significant changes in the abundance of Proteobacteria were observed. Overall, rAbBamA is a promising target, but specificity must be considered in the development of immunological tools against A. baumannii. (c) 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 17/10157-5 - Holobioma das esponjas marinhas, efeitos das mudanças climáticas e potenciais biotecnológicos: uma abordagem holística e multidisciplinar
• Beneficiário: Cristiane Cassiolato Pires Hardoim
• Modalidade de apoio: Bolsas no Brasil - Programa de Pesquisa sobre Mudanças Climáticas Globais - Jovens Pesquisadores