Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study

Autor(es)

Vitorio, Gabrielle dos Santos ^[1] ; de Almeida, Rainara Moreno Sanches ^[1] ; Pinto, Juliana Guerra ^[1] ; Fontana, Leticia Correa ^[1] ; Ferreira-Strixino, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Res & Dev, Lab Photobiol Appl Hlth, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 34, JUN 2021.
• Citações Web of Science: 0

Resumo

Gliosarcoma is an aggressive brain tumor. Photodynamic Therapy (PDT) is a treatment that can be used for various cancers of the CNS. The aim of this study was to analyze the effects of PDT with Photodithazine (PDZ) in the treatment of gliosarcoma, using 9 L/lacZ cells and serial concentrations of 200 mu g/mL to 3.1 mu g/mL of PDZ. The samples were divided into two groups: dark and light (10 J/cm2). The PDZ was internalized along all the cytoplasmic extension. Viability tests demonstrated a reduction in viable cells after PDT. The production of ROS was concentration-dependent and PDZ was found in mitochondria and lysosomes, presenting a discrete connection with alpha-tubulin. However, this structure is likely damaged, evidenced by changes in the morphological analysis. Thus, according to the parameters of this study, PDZ proved to be an interesting PS in PDT for the treatment of gliosarcoma, with the inherent limitations of an in vitro study. (AU)

• Processo FAPESP: 18/12274-1 - Avaliação da terapia fotodinâmica com Photodithazine no tratamento de gliossarcoma: estudo in vitro
• Beneficiário: Gabrielle dos Santos Vitorio
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/12211-4 - Estudo do efeito genotóxico, do potencial de membrana e das vias de morte celular da curcumina na terapia fotodinâmica em promastigota e amastigota de L. major e L. braziliensis: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/15302-6 - Análise morfológica e genotóxica do fotoenticine no tratamento de gliossarcoma
• Beneficiário: Rainara Moreno Sanches de Almeida
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica