Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma

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Silveira, Felipe Martins ^{[1, 2]} ; Schmidt, Tuany Rafaeli ^[3] ; Neumann, Bruna ^[3] ; Rosset, Clevia ^[4] ; Zanella, Virgilio Gonzales ^{[3, 5]} ; Maahs, Gerson Schulz ^[6] ; Trevizani Martins, Marco Antonio ^{[3, 7]} ; Arany, Praveen ^{[8, 9, 10, 11, 12, 13]} ; Wagner, Vivian Petersen ^[1] ; Lopes, Marcio Ajudarte ^[1] ; Santos-Silva, Alan Roger ^[1] ; Martins, Manoela Domingues ^{[3, 1, 2]}

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^[1] Univ Estadual Campinas, Oral Diag Dept, Piracicaba Dent Sch, Campinas - Brazil

^[2] Porto Alegre Clin Hosp, Expt Pathol Unit, Porto Alegre, RS - Brazil

^[3] Univ Fed Rio Grande do Sul, Sch Dent, Dept Oral Pathol, Porto Alegre, RS - Brazil

^[4] Porto Alegre Clin Hosp, Expt Res Ctr, Lab Res Unit, Porto Alegre, RS - Brazil

^[5] Santa Casa de Misericordia Porto Alegre, Santa Rita Hosp, Head & Neck Surg Dept, Porto Alegre, RS - Brazil

^[6] Porto Alegre Clin Hosp, Div Otorhinolaryngol, Porto Alegre, RS - Brazil

^[7] Porto Alegre Clin Hosp, Dept Oral Med, Porto Alegre, RS - Brazil

^[8] SUNY Buffalo, Dept Oral Biol, Sch Dent Med, Buffalo, NY - USA

^[9] SUNY Buffalo, Dept Oral Biol, Sch Engn, Buffalo, NY - USA

^[10] SUNY Buffalo, Dept Oral Biol, Sch Appl Sci, Buffalo, NY - USA

^[11] SUNY Buffalo, Dept Biomed Engn, Sch Dent Sci, Buffalo, NY - USA

^[12] SUNY Buffalo, Dept Biomed Engn, Sch Engn, Buffalo, NY - USA

^[13] SUNY Buffalo, Dept Biomed Engn, Sch Appl Sci, Buffalo, NY - USA

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; JUL 2021.
• Citações Web of Science: 0

Resumo

Background Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model. Methods BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm(3) (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm(2); 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis. Results No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups. Conclusion In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients. (AU)

• Processo FAPESP: 18/02233-6 - Segurança oncológica no uso da fotobiomodulação intraoral e extraoral em pacientes com carcinoma espinocelular de cavidade oral e orofaringe
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21785-4 - Relação da via de sinalização BDNF/TRKB com a agressividade e perfil de células tronco tumorais de neoplasias malignas de glândula salivar
• Beneficiário: Vivian Petersen Wagner
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado