genome sequence resource for the genus Passiflora, the genome of the wild diploid species Passiflora organensi

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Costa, Zirlane Portugal ^[1] ; Varani, Alessandro Mello ^[2] ; Cauz-Santos, Luiz Augusto ^{[3, 1]} ; Sader, Mariela Analia ^[4] ; Giopatto, Helena Augusto ^[5] ; Zirpoli, Bruna ^[4] ; Callot, Caroline ^[6] ; Cauet, Stephane ^[6] ; Marande, Willian ^[6] ; Souza Cardoso, Jessica Luana ^[1] ; Pinheiro, Daniel Guariz ; Kitajima, Joao Paulo ^[7] ; Dornelas, Marcelo Carnier ^[5] ; Harand, Andrea Pedrosa ; Berges, Helene ^[6] ; Monteiro-Vitorello, Claudia Barros ^[1] ; Carneiro Vieira, Maria Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Technol, BR-14884900 Jaboticabal - Brazil

^[3] Univ Vienna, Dept Bot & Biodivers Res, A-1030 Vienna - Austria

^[4] Univ Fed Pernambuco, Dept Bot, BR-50670901 Recife, PE - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas - Brazil

^[6] INRA, Ctr Natl Ressources Genom Veget, F-31326 Castanet Tolosan - France

^[7] Mendel Anal Genom AS, BR-04013000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT GENOME; v. 14, n. 3 JUL 2021.
• Citações Web of Science: 2

Resumo

The genus Passiflora comprises a large group of plants popularly known as passionfruit, much appreciated for their exotic flowers and edible fruits. The species (similar to 500) are morphologically variable (e.g., growth habit, size, and color of flowers) and are adapted to distinct tropical ecosystems. In this study, we generated the genome of the wild diploid species Passiflora organensis Gardner by adopting a hybrid assembly approach. Passiflora organensis has a small genome of 259 Mbp and a heterozygosity rate of 81%, consistent with its reproductive system. Most of the genome sequences could be integrated into its chromosomes with cytogenomic markers (satellite DNA) as references. The repeated sequences accounted for 58.55% of the total DNA analyzed, and the Tekay lineage was the prevalent retrotransposon. In total, 25,327 coding genes were predicted. Passiflora organensis retains 5,609 singletons and 15,671 gene families. We focused on the genes potentially involved in the locus determining self-incompatibility and the MADS-box gene family, allowing us to infer expansions and contractions within specific subfamilies. Finally, we recovered the organellar DNA. Structural rearrangements and two mitoviruses, besides relics of other mobile elements, were found in the chloroplast and mt-DNA molecules, respectively. This study presents the first draft genome assembly of a wild Passiflora species, providing a valuable sequence resource for genomic and evolutionary studies on the genus, and support for breeding cropped passionfruit species. (AU)

• Processo FAPESP: 18/21469-0 - Análise da expressão diferencial de genes durante a transição de fase em maracujazeiro (Passiflora spp.)
• Beneficiário: Marcelo Carnier Dornelas
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/11815-6 - Inferências filogenéticas em Passiflora com base na análise de genomas cloroplastidiais
• Beneficiário: Maria Lúcia Carneiro Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 19/07838-6 - Descobrindo o genoma de Passiflora organensis: uma referência em Passifloraceae e para estudos evolutivos
• Beneficiário: Zirlane Portugal da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/04216-9 - Genomas cloroplastidiais de passifloras: estrutura, inferências evolutivas e transferência de genes para o núcleo
• Beneficiário: Luiz Augusto Cauz dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/25242-0 - Caracterização e análise da expressão diferencial de genes envolvidos na transição de fase em maracujazeiro (Passiflora spp.)
• Beneficiário: Helena Augusto Gioppato
• Modalidade de apoio: Bolsas no Brasil - Doutorado