Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil

Autor(es)

da Silva, Patrick ^[1] ; Lustri, Bruna C. ^[1] ; Castilho, Ivana Giovannetti ^[2] ; Ferreira, Adriano Martison ^[3] ; Hernandes, Rodrigo T. ^[2] ; Schembri, Mark A. ^[4] ; Moreira, Cristiano G. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Med, Clin Anal Lab, Botucatu, SP - Brazil

^[4] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld - Australia

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 52, n. 3, p. 1067-1075, SEP 2021.
• Citações Web of Science: 0

Resumo

Urinary tract infections (UTIs) are a major public health concern in both community and hospital settings worldwide. Uropathogenic Escherichia coli (UPEC) is the main causative agent of UTI and increasingly associated with antibiotic resistance. Herein, we report the draft genome sequence of 9 fluoroquinolone-resistant UPEC isolates from Brazil and examine selected major phenotypic features, such as antimicrobial resistance profile, phylogroup, serotype, sequence type (ST), virulence genes, and resistance marks. Besides the quinolone resistance, beta-lactams, ESBL production, aminoglycosides, and tetracycline resistance were observed. High prevalence of 20 virulence genes was detected in all isolates, such as those encoding type 1 fimbriae, acid tolerance system, and hemolysin E, particularly within E. coli B2 phylogroup, as ST131 and ST1193 strains, among other genomic analyses as genomic islands, resistance plasmids, and integron identification. (AU)

• Processo FAPESP: 14/06779-2 - Investigação do papel da sinalização química e de mecanismos auxiliares na virulência de Salmonella enterica sorovar Typhimurium e outros enteropatógenos
• Beneficiário: Cristiano Gallina Moreira
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 19/03049-7 - Transcriptoma da sinalização química bacteriana em Salmonella Typhimurium, investigação de enteropatógenos bacterianos globais emergentes em São Paulo e emprego de modelo alternativo de infecção no estudo da patogênese.
• Beneficiário: Cristiano Gallina Moreira
• Linha de fomento: Auxílio à Pesquisa - Regular